324870 | Anti-α-Epithelial Cell Sodium Channel (20-42) Rabbit pAb

324870
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      324870-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~97 kDa glycosylated α-ENaC protein.
          Catalogue Number324870
          Brand Family Calbiochem®
          SynonymsAnti-α-ENaC
          References
          ReferencesBenos, D.J., and Stanton, B.A. 1999. J. Physiol. London 520, 631.
          Mano, I., and Driscoll, M. 1999. Bioessays 21, 568.
          Fyfe, G.K., et al. 1998. Semin. Nephrol. 18, 138.
          Product Information
          FormLiquid
          FormulationIn PBS, 1 mg/ml BSA.
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunoprecipitation
          Application NotesImmunoblotting (1 µg/ml)
          Immunoprecipitation (see comments)
          Application CommentsDoes not cross-react with β-ENaC or γ-ENaC. Also reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
          Biological Information
          Immunogena synthetic peptide [LMKGNKREEQGLGPEPAAPQQPT(C)] corresponding to amino acids 20-42 of human α-epithelial cell sodium channel (α-ENaC), conjugated to carrier protein
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Rat
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          324870

          References

          Reference overview
          Benos, D.J., and Stanton, B.A. 1999. J. Physiol. London 520, 631.
          Mano, I., and Driscoll, M. 1999. Bioessays 21, 568.
          Fyfe, G.K., et al. 1998. Semin. Nephrol. 18, 138.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-August-2007 RFH
          SynonymsAnti-α-ENaC
          ApplicationImmunoblotting (1 µg/ml)
          Immunoprecipitation (see comments)
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~97 kDa glycosylated α-ENaC protein.
          BackgroundEpithelial sodium channels are amiloride-sensitive members of the degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels. ENaC in the kidney, lung, and colon plays a role in trans-epithelial Na+ and fluid balance. ENaC also mediates aldosterone-dependent sodium reabsorption in the distal nephron of the kidney, thus regulating blood pressure. ENaC is believed to be regulated through association with the cystic fibrosis transmembrane conductance regulator (CFTR) chloride ion channel.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic peptide [LMKGNKREEQGLGPEPAAPQQPT(C)] corresponding to amino acids 20-42 of human α-epithelial cell sodium channel (α-ENaC), conjugated to carrier protein
          IsotypeIgG
          Specieshuman, rat
          FormLiquid
          FormulationIn PBS, 1 mg/ml BSA.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsDoes not cross-react with β-ENaC or γ-ENaC. Also reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesBenos, D.J., and Stanton, B.A. 1999. J. Physiol. London 520, 631.
          Mano, I., and Driscoll, M. 1999. Bioessays 21, 568.
          Fyfe, G.K., et al. 1998. Semin. Nephrol. 18, 138.