GR13 Anti-EGFR (Ab-3) Mouse mAb (225)

GR13
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      GR13-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~170 kDa epidermal growth factor receptor protein. For neutralization studies Cat. No. GR13L is recommended.
          Catalogue NumberGR13
          Brand Family Calbiochem®
          SynonymsAnti- Epidermal Growth Factor Receptor
          References
          ReferencesHunter T. 1984. Nature 311, 414.
          Reynolds, F.H., et. al 1981. Nature 292, 259.
          Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
          Product Information
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Negative controlHL-60 cells
          Positive controlA-431 cells
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesOriginal Clone Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7755. Kawamoto, T., et al. 1984. J. Biol. Chem. 259, 7761. Masui, H., et al. 1984. Cancer Res. 44, 1002. Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Biol. Med. 1, 511.
          Key Applications Immunoprecipitation
          Application NotesImmunoprecipitation (1-2 µg per sample)
          Neutralization Studies (2 µg/ml, see application references; use Cat. No. GR13L)
          Application CommentsShown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. For neutralization studies GR13L is recommended. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenpartially purified, human EGFR from A431 cells
          ImmunogenHuman
          Clone225
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-EGFR (Ab-3) Mouse mAb (225) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-EGFR (Ab-3) Mouse mAb (225) Certificates of Analysis

          TitleLot Number
          GR13

          References

          Reference overview
          Hunter T. 1984. Nature 311, 414.
          Reynolds, F.H., et. al 1981. Nature 292, 259.
          Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision25-October-2007 JSW
          SynonymsAnti- Epidermal Growth Factor Receptor
          ApplicationImmunoprecipitation (1-2 µg per sample)
          Neutralization Studies (2 µg/ml, see application references; use Cat. No. GR13L)
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa epidermal growth factor receptor protein.
          BackgroundEpidermal growth factor (EGF) initiates its effects on cell growth through interaction with a 170,000 dalton cell surface glycoprotein receptor. Binding of EGF or transforming growth factor a to the receptor activates a tyrosine-specific protein kinase intrinsic to the EGF receptor protein. The phosphotyrosine content of target cells increases rapidly and the EGF receptor is phosphorylated on tyrosine residues. Subsequent to binding, EGF receptor complexes are internalized and cell proliferation takes place with partially-purified EGF receptor from human A431 epidermal carcinoma cells.
          HostMouse
          Immunogen speciesHuman
          Immunogenpartially purified, human EGFR from A431 cells
          Clone225
          IsotypeIgG₁
          Specieshuman, not mouse, not rat
          Positive controlA-431 cells
          Negative controlHL-60 cells
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsShown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. For neutralization studies GR13L is recommended. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesHunter T. 1984. Nature 311, 414.
          Reynolds, F.H., et. al 1981. Nature 292, 259.
          Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
          Application referencesOriginal Clone Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7755. Kawamoto, T., et al. 1984. J. Biol. Chem. 259, 7761. Masui, H., et al. 1984. Cancer Res. 44, 1002. Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Biol. Med. 1, 511.

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          Categories

          Life Science Research > Antibodies and Assays > Primary Antibodies