324390 | Anti-Dopamine D₁ Receptor Rabbit pAb

324390
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      324390-100UL
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          Plastic ampoule 100 ul
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          Description
          OverviewRecognizes the dopamine D1 receptor in rat brain. Does not cross-react with other dopamine receptors.
          Catalogue Number324390
          Brand Family Calbiochem®
          References
          ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
          Product Information
          FormLyophilized
          FormulationUndiluted serum.
          Positive controlRat brain
          PreservativeNone
          Applications
          Application ReferencesImmunoblotting and Immunohistochemistry Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325. Species Reactivity Kipnis, J., et al. 2004. Journal of Neuroscience 24 6133.
          Key Applications Immunoblotting (Western Blotting)
          Not Paraffin Sections
          Enzyme-Linked Immunosorbent Assay
          Free-floating Sections
          Frozen Sections
          Application NotesELISA (see comments)
          Immunoblotting (1:1000)
          Frozen Sections (1:5000)
          Free-Floating Sections (1:5000)
          Paraffin Sections (not recommended)
          Application CommentsThis antibody has also been reported to work in ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For frozen sections a 1:5000 dilution of the reconstituted serum demonstrates specific staining of rat brain sections including the medial septum, nucleus accumbens, dentate gyrus, globus pallidus, medial forebrain bundle, cortex regions 1-3, substantia nigra reticulata, and the ventral tegmental area. Strong nuclear staining of oligodendrocytes was also observed. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena synthetic peptide (MDGTGLVVERDFSC) corresponding to amino acids 9-21 of human D₁dopamine receptor
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsReconstitute the antibody with 100 µl PBS containing 1% BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          324390

          References

          Reference overview
          Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Alzheimer's Disease Brochure & Technical Guide

          Citations

          Title
        • Jonathan Kipnis, et al. (2004) Dopamine, through the Extracellular Signal-Related Kinase Pathway, Downregulates CD4+CD25+ Regulatory T-Cell Activity: Implications for Neurodegeneration. Journal of Neuroscience 24, 6133-6143.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision27-July-2007 RFH
          ApplicationELISA (see comments)
          Immunoblotting (1:1000)
          Frozen Sections (1:5000)
          Free-Floating Sections (1:5000)
          Paraffin Sections (not recommended)
          DescriptionRabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~50 kDa dopamine D1 receptor protein.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic peptide (MDGTGLVVERDFSC) corresponding to amino acids 9-21 of human D₁dopamine receptor
          IsotypeIgG
          Specieshuman, mouse, rat
          Positive controlRat brain
          FormLyophilized
          FormulationUndiluted serum.
          PreservativeNone
          CommentsThis antibody has also been reported to work in ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For frozen sections a 1:5000 dilution of the reconstituted serum demonstrates specific staining of rat brain sections including the medial septum, nucleus accumbens, dentate gyrus, globus pallidus, medial forebrain bundle, cortex regions 1-3, substantia nigra reticulata, and the ventral tegmental area. Strong nuclear staining of oligodendrocytes was also observed. Variables associated with assay conditions will dictate the proper working dilution.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute the antibody with 100 µl PBS containing 1% BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
          Toxicity Standard Handling
          ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
          Citation
        • Jonathan Kipnis, et al. (2004) Dopamine, through the Extracellular Signal-Related Kinase Pathway, Downregulates CD4+CD25+ Regulatory T-Cell Activity: Implications for Neurodegeneration. Journal of Neuroscience 24, 6133-6143.
        • Application referencesImmunoblotting and Immunohistochemistry Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325. Species Reactivity Kipnis, J., et al. 2004. Journal of Neuroscience 24 6133.