MAB5270-100UG Anti-Choline Acetyltransferase Antibody, clone 1.B3.9B3

MAB5270-100UG
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Po, R ELISA, IHC, IH(P), WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB5270-100UG
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Choline Acetyltransferase Antibody, clone 1.B3.9B3
      Alternate Names
      • ChAT
      References
      Product Information
      FormatPurified
      Control
      • Brain tissue
      PresentationPurified immunoglobulin. Lyophilized from 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide. Reconstitute to 100 μg/mL with sterile distilled water.
      Applications
      ApplicationThis Anti-Choline Acetyltransferase Antibody, clone 1.B3.9B3 is validated for use in ELISA, IH, IH(P), WB for the detection of Choline Acetyltransferase.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern blot

      ELISA

      Immunohistochemistry: 10-20 μg/mL in paraffin embedded sections {See Ratcliffe et al. 1998}

      Optimal working dilutions and protocols must be determined by end user.
      Biological Information
      ImmunogenHighly purified ChAT obtained from porcine brain (Ostermann-Fatif et al., 1990).
      Clone1.B3.9B3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with choline acetyltransferase (ChAT)-positive cells from humans, rats and pigs (Ostermann-Fatif et al., 1992a,b).
      IsotypeIgG1
      Species Reactivity
      • Human
      • Pig
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.
      Gene Symbol
      • CHAT
      • ChAT
      • CMS1A2
      • CHOACTase
      • CMS1A
      • EC 2.3.1.6
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
      SIZE: 748 amino acids; 82568 Da
      DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
      SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
      Molecular Weight69 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized antibody at 2–8°C in undiluted aliquots for up to 6 months. Maintain reconstituted antibody at -20°C in undiluted aliquots for up to 6 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Choline Acetyltransferase Antibody, clone 1.B3.9B3 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Choline Acetyltransferase Antibody, clone 1.B3.9B3 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-CHOLINE ACETYLTRANSFERASE2482235
      MOUSE ANTI-CHOLINE ACETYLTRANSFERASE - 31735413173541
      MOUSE ANTI-CHOLINE ACETYLTRANSFERASE -28511742851174
      MOUSE ANTI-CHOLINE ACETYLTRANSFERASE MONOCLONAL ANTI2881076
      MOUSE ANTI-CHOLINE ACETYLTRANSFERASE MONOCLONAL ANTIBODY2990217
      MOUSE ANTI-CHOLINE ACETYLTRANSFERASE MONOCLONAL ANTIBODY - 23956982395698
      MOUSE ANTI-CHOLINE ACETYLTRANSFERASE MONOCLONAL ANTIBODY - 24500582450058
      MOUSE ANTI-CHOLINE ACETYLTRANSFERASE MONOCLONAL ANTIBODY -25042942504294

      References

      Reference overviewPub Med ID
      An efficient sandwich-ELISA for the determination of choline acetyltransferase.
      Ostermann-Latif, C, et al.
      J. Immunol. Methods, 157: 73-9 (1993)  1993

      Show Abstract
      8423376 8423376
      Large-scale purification of choline acetyltransferase and production of highly specific antisera.
      Ostermann, C, et al.
      Eur. J. Biochem., 192: 215-8 (1990)  1990

      Show Abstract
      2401292 2401292
      Use of cryoprotectant to maintain long-term peptide immunoreactivity and tissue morphology.
      Watson, R E, et al.
      Peptides, 7: 155-9 (1986)  1986

      Show Abstract
      3520509 3520509
      Organization and morphological characteristics of cholinergic neurons: an immunocytochemical study with a monoclonal antibody to choline acetyltransferase.
      Houser, C R, et al.
      Brain Res., 266: 97-119 (1983)  1983

      Show Abstract
      6850348 6850348
      Production of specific antisera and monoclonal antibodies to choline acetyltransferase: characterization and use for identification of cholinergic neurons.
      Eckenstein, F and Thoenen, H
      EMBO J., 1: 363-8 (1982)  1982

      Show Abstract
      6821323 6821323

      Data Sheet

      Title
      MOUSE ANTI-CHOLINE ACETYLTRANSFERASE MONOCLONAL ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies