209550 | Anti-Cannabinoid Receptor CB₁ (1-77) Rabbit pAb

209550
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Ch, F, Gr, H, Mk, M, R, Sal Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      209550-100UL
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          Plastic ampoule 100 ul
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          Description
          OverviewRecognizes the ~60 kDa CB1 receptor protein.
          Catalogue Number209550
          Brand Family Calbiochem®
          Application Data
          Detection of rat cannabinoid receptor 1 by immunohistochemistry. Sample: Rat hippocampus tissue. Primary antibody: Anti-Cannabinoid Receptor CB1 (1-77) Rabbit pAb (Cat. No. 209550) (1:1000). Detection: fluorescence.

          Detection of chicken cannabinoid receptor 1 by immunohistochemistry. Sample: Transverse spinal cord sections of E6 chick embryo. Primary antibody: Anti-Cannabinoid Receptor CB1 (1-77) Rabbit pAb (Cat. No. 209550) (Dilution Assay Dependent). Detection: fluorescence.
          References
          Product Information
          FormLiquid
          FormulationIn PBS, 1 mg/ml BSA, 50% glycerol.
          Positive controlHippocampal neurons
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesSpecies Cross-Reactivity

          Schomacher, M., et al. 2006. Acta Neuropathol. 111, 8.
          Small-Howard, A.L., et al. 2005. Biochem. J. 388, 465.
          Bouchard, J.F., 2003. Life Sci. 72, 1859.
          Key Applications Immunoblotting (Western Blotting)
          Immunocytochemistry
          Paraffin Sections
          Application NotesImmunoblotting (1:1000)
          Immunocytochemistry (1:100-1:1000)
          Paraffin Sections (see comments)
          Application CommentsMay also recognize weaker bands at ~23 kDa, ~72 kDa, and ~180 kDa in some tissues. Immunocytochemical staining of living or fixed cultured hippocampal neurons show intense, punctate staining along processes with little staining over cell bodies. Variables associated with assay conditions will dictate the optimal working dilution.
          Biological Information
          Immunogena recombinant protein consisting of the first 77 amino acids of rat CB₁ receptor
          ImmunogenRat
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Chicken
          • Fish
          • Gerbil
          • Human
          • Monkey
          • Mouse
          • Rat
          • Salamander
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          209550

          Brochure

          Title
          Antibody Sourcebook!" Second Edition

          Citations

          Title
        • Bouchard, J.F., 2003. Life Sci. 72, 1859.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision06-October-2010 RFH
          ApplicationImmunoblotting (1:1000)
          Immunocytochemistry (1:100-1:1000)
          Paraffin Sections (see comments)
          Application Data
          Detection of rat cannabinoid receptor 1 by immunohistochemistry. Sample: Rat hippocampus tissue. Primary antibody: Anti-Cannabinoid Receptor CB1 (1-77) Rabbit pAb (Cat. No. 209550) (1:1000). Detection: fluorescence.

          Detection of chicken cannabinoid receptor 1 by immunohistochemistry. Sample: Transverse spinal cord sections of E6 chick embryo. Primary antibody: Anti-Cannabinoid Receptor CB1 (1-77) Rabbit pAb (Cat. No. 209550) (Dilution Assay Dependent). Detection: fluorescence.
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~60 kDa CB1 receptor protein.
          BackgroundCannabinoids exert their physiological effects through two G-protein coupled receptors, CB1 and CB2. Both receptors inhibit adenylyl cyclase and stimulate MAP kinase. CB1 receptors also modulate ion channels through direct G protein interactions. D-9-tetrahydrocannibinol and related ligands exert their psychoactive effects by inhibiting presynaptic N- and P/Q-type calcium channels. CB1 receptors are primarily expressed in brain (particularly cortex, striatum, hippocampus and cerebellum), spinal cord, eye, and testis. CB1 receptors are involved in the modulation of nociception, movement, learning and memory, emotion, and many other neuronal processes.
          HostRabbit
          Immunogen speciesRat
          Immunogena recombinant protein consisting of the first 77 amino acids of rat CB₁ receptor
          IsotypeIgG
          Specieschicken, fish, gerbil, human, monkey, mouse, rat, salamander
          Positive controlHippocampal neurons
          FormLiquid
          FormulationIn PBS, 1 mg/ml BSA, 50% glycerol.
          Preservative≤0.1% sodium azide
          CommentsMay also recognize weaker bands at ~23 kDa, ~72 kDa, and ~180 kDa in some tissues. Immunocytochemical staining of living or fixed cultured hippocampal neurons show intense, punctate staining along processes with little staining over cell bodies. Variables associated with assay conditions will dictate the optimal working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          Citation
        • Bouchard, J.F., 2003. Life Sci. 72, 1859.
        • Application referencesSpecies Cross-Reactivity

          Schomacher, M., et al. 2006. Acta Neuropathol. 111, 8.
          Small-Howard, A.L., et al. 2005. Biochem. J. 388, 465.
          Bouchard, J.F., 2003. Life Sci. 72, 1859.