208910 | Anti-Calreticulin (405-417) Rabbit pAb

208910
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      B, Ca, Ht, H, Mk, M, Porcine, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      208910-50UL
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          Plastic ampoule 50 ul
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          Description
          OverviewRecognizes the ~63 kDa calreticulin protein in heat-shocked HeLa cells.
          Catalogue Number208910
          Brand Family Calbiochem®
          Application Data
          Detection of human calreticulin by immunoblotting. Samples: Molecular weight marker (lane 1), whole cell lysates from HeLa cells (lane 2), L929 cells (lane 3), and Rat2 cells (lane 4). Primary antibody: Anti-Calreticulin (405-417) Rabbit pAb (Cat. No. 208910) (2 µg/ml). Detection: chemiluminescence.
          References
          ReferencesJanicki, S., et al. 1997. J. Cell Biol. 139, 485.
          Wada, I., et al. 1997. EMBO J. 16, 5420.
          Nauseef, W.F., et al. 1995. J. Biol. Chem. 270, 4741.
          Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
          Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.
          Product Information
          FormLiquid
          FormulationIn PBS, 50% glycerol, pH 7.2.
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoprecipitation
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Immunohistochemistry
          Application NotesImmunoblotting (1:500, mouse/rat; 1:1000, human, chemiluminescent)
          Immunocytochemistry (see comments)
          Immunoprecipitation (see comments)
          Immunohistochemistry (see comments)
          Application CommentsDoes not recognize calreticulin in Drosophila cells. This antibody has been reported to work for immunocytochemistry, immunohistochemistry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
          Biological Information
          Immunogena synthetic peptide [(C)EEEDVPGQAKDEL] corresponding to amino acids 405-417 of human calreticulin, conjugated to KLH
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Bovine
          • Canine
          • Hamster
          • Human
          • Monkey
          • Mouse
          • Porcine
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          208910

          References

          Reference overview
          Janicki, S., et al. 1997. J. Cell Biol. 139, 485.
          Wada, I., et al. 1997. EMBO J. 16, 5420.
          Nauseef, W.F., et al. 1995. J. Biol. Chem. 270, 4741.
          Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
          Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision20-April-2009 JSW
          ApplicationImmunoblotting (1:500, mouse/rat; 1:1000, human, chemiluminescent)
          Immunocytochemistry (see comments)
          Immunoprecipitation (see comments)
          Immunohistochemistry (see comments)
          Application Data
          Detection of human calreticulin by immunoblotting. Samples: Molecular weight marker (lane 1), whole cell lysates from HeLa cells (lane 2), L929 cells (lane 3), and Rat2 cells (lane 4). Primary antibody: Anti-Calreticulin (405-417) Rabbit pAb (Cat. No. 208910) (2 µg/ml). Detection: chemiluminescence.
          DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~63 kDa calreticulin protein.
          BackgroundCalreticulin is a calcium binding protein found primarily in the ER and the sarcoplasmic reticulum, although it also has been detected in the nucleus and nuclear envelope. It has the conserved KDEL sequence at its C-terminus for retention in the ER. Calreticulin, which contains both high and low affinity calcium binding sites, is involved in calcium storage and regulation. The calcium binding at the C-terminus of calreticulin has been postulated to be involved with regulating the interaction of KDEL proteins with the KDEL receptor. Calreticulin also acts as a molecular chaperone for protein folding, in concert with Grp78 and Grp94.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic peptide [(C)EEEDVPGQAKDEL] corresponding to amino acids 405-417 of human calreticulin, conjugated to KLH
          IsotypeIgG
          Speciesbovine, canine, hamster, human, monkey, mouse, porcine, rat
          FormLiquid
          FormulationIn PBS, 50% glycerol, pH 7.2.
          Preservative≤0.1% sodium azide
          CommentsDoes not recognize calreticulin in Drosophila cells. This antibody has been reported to work for immunocytochemistry, immunohistochemistry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesJanicki, S., et al. 1997. J. Cell Biol. 139, 485.
          Wada, I., et al. 1997. EMBO J. 16, 5420.
          Nauseef, W.F., et al. 1995. J. Biol. Chem. 270, 4741.
          Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
          Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.