PC253L Anti-Calbindin D-28K (Ab-1) Rabbit pAb

PC253L
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service
      View Pricing & Availability

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Bab, H, Mk, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PC253L-100UL
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Plastic ampoule 100 ul
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewRecognizes the Calbindin D-28K protein in rat hippocampus tissue.
          Catalogue NumberPC253L
          Brand Family Calbiochem®
          References
          ReferencesConde, F., et al. 1994. J. Comp. Neurol. 341, 95.
          Heizmann, C.W. 1993. Acta Neurobiol. Exp. 53, 15.
          Baimbridge, K.G., et al. 1992. Trends Neurosci. 15, 303.
          Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci. 16, 98.
          Product Information
          FormLyophilized
          FormulationUndiluted serum.
          Positive controlRat striatum, hippocampus, or cortex
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesFrozen and Vibrotome Sections Raven, M.A., et al. 2005. Vis. Neurosci. 22, 569.
          Key Applications Frozen Sections
          T Cell Co-stimulation
          Application NotesFrozen Sections (whole mount/vibrotome sections, 1:1000, fluorescence)
          Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, see application references)
          Application CommentsAntibody specificity was examined in the rat striatum, cortex, and hippocampus. Detection of primary antibody with biotin-streptavidin/peroxidase reagents according to manufacturers directions. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenpurified bovine cerebellum calbindin D-28K protein
          ImmunogenBovine
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Baboon
          • Human
          • Monkey
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution will contain ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Calbindin D-28K (Ab-1) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Calbindin D-28K (Ab-1) Rabbit pAb Certificates of Analysis

          TitleLot Number
          PC253L

          References

          Reference overview
          Conde, F., et al. 1994. J. Comp. Neurol. 341, 95.
          Heizmann, C.W. 1993. Acta Neurobiol. Exp. 53, 15.
          Baimbridge, K.G., et al. 1992. Trends Neurosci. 15, 303.
          Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci. 16, 98.

          Citations

          Title
        • Mary. A. Raven, et al. (2005) Developmental Improvement in the Regularity and Packing of Mouse Horizontal Cells: Implications for Mechanisms Underlying Mosaic Pattern Formation. Visual Neuroscience 22, 569-573.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision09-October-2007 RFH
          ApplicationFrozen Sections (whole mount/vibrotome sections, 1:1000, fluorescence)
          Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, see application references)
          DescriptionRabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes the calbindin D-28K protein.
          BackgroundSeveral EF-hand calcium-binding proteins have been identified within the nervous system, which includes calbindin, calretinin, and parvalbumin. These cytosolic calcium-binding proteins modulate and mediate the function of the intracellular second messenger calcium. These proteins are believed to function in the buffering, transport and regulation of calcium. Calbindin, calretinin, and parvalbumin are localized in distinct neuronal subpopulations, and antibodies against these proteins are useful markers for neuranatomical and developmental studies.
          HostRabbit
          Immunogen speciesBovine
          Immunogenpurified bovine cerebellum calbindin D-28K protein
          IsotypeIgG
          Speciesbaboon, human, monkey, rat
          Positive controlRat striatum, hippocampus, or cortex
          FormLyophilized
          FormulationUndiluted serum.
          Preservative≤0.1% sodium azide
          CommentsAntibody specificity was examined in the rat striatum, cortex, and hippocampus. Detection of primary antibody with biotin-streptavidin/peroxidase reagents according to manufacturers directions. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution will contain ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling
          ReferencesConde, F., et al. 1994. J. Comp. Neurol. 341, 95.
          Heizmann, C.W. 1993. Acta Neurobiol. Exp. 53, 15.
          Baimbridge, K.G., et al. 1992. Trends Neurosci. 15, 303.
          Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci. 16, 98.
          Citation
        • Mary. A. Raven, et al. (2005) Developmental Improvement in the Regularity and Packing of Mouse Horizontal Cells: Implications for Mechanisms Underlying Mosaic Pattern Formation. Visual Neuroscience 22, 569-573.
        • Application referencesFrozen and Vibrotome Sections Raven, M.A., et al. 2005. Vis. Neurosci. 22, 569.