CB1046 Anti-CLDN1 Mouse mAb (1C5-D9)

CB1046
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      CB1046-100UG
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      Discontinued
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      Available
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          Contact Customer Service
          Contact Customer Service

          100 μg
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          Description
          OverviewRecognizes the ~17 kDa CLDN1 protein in PRC/PRF/5 and A431 cells and human small intestine and colon tissue.
          Catalogue NumberCB1046
          Brand Family Calbiochem®
          SynonymsAnti-Claudin 1
          Application Data
          Detection of human CLDN1 by immunoblotting. Sample: Whole cell lysate from PRC/PRF/5 cells. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human CLDN1 by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human CLDN1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (3 µg/ml). Detection: colorimetric (DAB).

          Detection of human CLDN1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human colon tissue. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (5 µg/ml). Detection: colorimetric (DAB).
          References
          ReferencesGuevin C, et al. 2009. Antiviral Res. 84, 14.
          Sainz, B., Jr, et al. 2009. Virol. J. 6, 103.
          Stamataki Z, et al. 2009. Blood 113 585.
          Farquhar MJ, et al. 2008. J. Virol. 82 8797.
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Negative control293T cells
          Positive controlPRC/PRF/5 cells, A431 cells, Human small intestine tissue, human colon tissue
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Paraffin Sections
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Paraffin Sections (3-5 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenFull-length, recombinant, human CLDN1 (aa 1-212), expressed as a GST fusion protein
          Clone1C5-D9
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-CLDN1 Mouse mAb (1C5-D9) SDS

          Title

          Safety Data Sheet (SDS) 

          References

          Reference overview
          Guevin C, et al. 2009. Antiviral Res. 84, 14.
          Sainz, B., Jr, et al. 2009. Virol. J. 6, 103.
          Stamataki Z, et al. 2009. Blood 113 585.
          Farquhar MJ, et al. 2008. J. Virol. 82 8797.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-November-2010 RFH
          SynonymsAnti-Claudin 1
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Paraffin Sections (3-5 µg/ml)
          Application Data
          Detection of human CLDN1 by immunoblotting. Sample: Whole cell lysate from PRC/PRF/5 cells. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human CLDN1 by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human CLDN1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (3 µg/ml). Detection: colorimetric (DAB).

          Detection of human CLDN1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human colon tissue. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (5 µg/ml). Detection: colorimetric (DAB).
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~17 kDa CLDN1 protein.
          BackgroundCLDN1 is a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations results in neonatal ichthyosis-sclerosing cholangitis syndrome.
          HostMouse
          ImmunogenFull-length, recombinant, human CLDN1 (aa 1-212), expressed as a GST fusion protein
          Clone1C5-D9
          IsotypeIgG2a
          Specieshuman
          Positive controlPRC/PRF/5 cells, A431 cells, Human small intestine tissue, human colon tissue
          Negative control293T cells
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review
          ReferencesGuevin C, et al. 2009. Antiviral Res. 84, 14.
          Sainz, B., Jr, et al. 2009. Virol. J. 6, 103.
          Stamataki Z, et al. 2009. Blood 113 585.
          Farquhar MJ, et al. 2008. J. Virol. 82 8797.