217620 Anti-CD64 Mouse mAb (10.1)

217620
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service
      View Pricing & Availability
      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      217620-100UG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Plastic ampoule 100 μg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewRecognizes the ~72 kDa CD64 protein (FcR1 receptor).
          Catalogue Number217620
          Brand Family Calbiochem®
          References
          ReferencesDougherty, G.J., et al. 1987. Eur. J. Immunol. 17, 1453.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
          Negative controlHPB-MLT
          Positive control4937 cells
          Preservative0.5 mg/ml gentamycin sulfate
          Applications
          Key Applications Immunoprecipitation
          Neutralization Studies
          Flow Cytometry
          Frozen Sections
          Immunoblotting (Western Blotting)
          Application NotesFlow Cytometry (10 µg/ml)
          Frozen Sections (see comments)
          Immunoblotting (see comments)
          Immunoprecipitation (see comments)
          Neutralization Studies (see comments)
          Application CommentsThe antibody is reported to block immunoglobulin binding to FcR1. FACS was performed on 5x105 cultured U-937 human tumor cells and analyzed by flow cytometry. Optimal binding concentration tested was 10 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogenhuman rheumatoid synovial fluid cells/monocytes
          ImmunogenHuman
          Clone10.1
          HostMouse
          Purity≥95% by SDS-PAGE
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          217620

          References

          Reference overview
          Dougherty, G.J., et al. 1987. Eur. J. Immunol. 17, 1453.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision25-July-2007 RFH
          ApplicationFlow Cytometry (10 µg/ml)
          Frozen Sections (see comments)
          Immunoblotting (see comments)
          Immunoprecipitation (see comments)
          Neutralization Studies (see comments)
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~72 kDa CD64 (FcR1) protein.
          HostMouse
          Immunogen speciesHuman
          Immunogenhuman rheumatoid synovial fluid cells/monocytes
          Clone10.1
          IsotypeIgG₁
          Specieshuman
          Positive control4937 cells
          Negative controlHPB-MLT
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Purity≥95% by SDS-PAGE
          Preservative0.5 mg/ml gentamycin sulfate
          CommentsThe antibody is reported to block immunoglobulin binding to FcR1. FACS was performed on 5x105 cultured U-937 human tumor cells and analyzed by flow cytometry. Optimal binding concentration tested was 10 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
          Toxicity Standard Handling
          ReferencesDougherty, G.J., et al. 1987. Eur. J. Immunol. 17, 1453.