217605 | Anti-CD54 Mouse mAb (15.2)

217605
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      View Pricing & Availability
      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      217605-100UG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Plastic ampoule 100 μg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewRecognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1) in Raji cells.
          Catalogue Number217605
          Brand Family Calbiochem®
          SynonymsAnti-ICAM-1, Domain 1
          References
          ReferencesReilly, P.L., et al. 1995. J. Immunol. 155, 529.
          Berendt, A.R., et al. 1992. Cell 68, 71.
          Dransfield, I., et al. 1992. J. Cell Biol. 116, 1527.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
          Positive controlRaji cells
          Preservative0.5 mg/ml gentamicin sulfate
          Applications
          Key Applications Immunoprecipitation
          Neutralization Studies
          Flow Cytometry
          Frozen Sections
          Immunoblotting (Western Blotting)
          Application NotesFlow Cytometry (1-10 µg/ml)
          Frozen Sections (see comments)
          Immunoblotting (see comments)
          Immunoprecipitation (see comments)
          Neutralization Studies (see comments)
          Application CommentsFACS was performed on 5x105 cultured Raji human tumor cells using 1 µg/ml Anti-CD54 and FITC-conjugated goat anti-mouse IgG. This antibody will inhibit CD54 binding to LFA-1. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogenhuman monocytes
          ImmunogenHuman
          Clone15.2
          HostMouse
          Purity≥95% by SDS-PAGE
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          217605

          References

          Reference overview
          Reilly, P.L., et al. 1995. J. Immunol. 155, 529.
          Berendt, A.R., et al. 1992. Cell 68, 71.
          Dransfield, I., et al. 1992. J. Cell Biol. 116, 1527.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision25-July-2007 RFH
          SynonymsAnti-ICAM-1, Domain 1
          ApplicationFlow Cytometry (1-10 µg/ml)
          Frozen Sections (see comments)
          Immunoblotting (see comments)
          Immunoprecipitation (see comments)
          Neutralization Studies (see comments)
          DescriptionProtein A purified mouse monoclonal antibody. Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1).
          BackgroundHuman CD54 (Intercellular Adhesion Molecule-1, or ICAM-1) is a ~90 kDa lymphocyte/monocyte cell surface glycoprotein that mediates cell-cell adhesion by interacting with the integrins LFA-1 (CD11a/CD18) and MAC-1 (CD11b/CD18).
          HostMouse
          Immunogen speciesHuman
          Immunogenhuman monocytes
          Clone15.2
          IsotypeIgG₁
          Specieshuman
          Positive controlRaji cells
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
          Purity≥95% by SDS-PAGE
          Preservative0.5 mg/ml gentamicin sulfate
          CommentsFACS was performed on 5x105 cultured Raji human tumor cells using 1 µg/ml Anti-CD54 and FITC-conjugated goat anti-mouse IgG. This antibody will inhibit CD54 binding to LFA-1. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
          Toxicity Standard Handling
          ReferencesReilly, P.L., et al. 1995. J. Immunol. 155, 529.
          Berendt, A.R., et al. 1992. Cell 68, 71.
          Dransfield, I., et al. 1992. J. Cell Biol. 116, 1527.