217669 Anti-CD28 Mouse mAb (ANC28.1/5D10)

217669
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      217669-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes CD28 on human T cells and BHK cells transfected with human CD28.
          Catalogue Number217669
          Brand Family Calbiochem®
          References
          ReferencesKariv, I., et al. 1996. J. Immunol. 157, 29.
          61st Forum in Immunology. 1995. Res. Immunol. 146, 127.
          Jenkins, M.K., and Johnson, J.G. 1993. Curr. Opin. Immunol. 5, 361.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
          Preservative500 µg/ml gentamycin sulfate
          Applications
          Key Applications T Cell Co-stimulation
          Flow Cytometry
          Frozen Sections
          Immunoprecipitation
          Application NotesFlow Cytometry (5 µg/ml)
          Frozen Sections (see comments)
          Immunoprecipitation (see comments)
          T cell Co-stimulation (see comments)
          Application CommentsMonomeric and cross-linked antibody will stimulate expression of IL-2 from CD28+ cells. FACS was performed on 5x105 cultured HPB-MLT cells using anti-CD28 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogenhuman CD28 Ig fusion protein and HPB-MLT human tumor cells
          ImmunogenHuman
          CloneANC28.1/5D10
          HostMouse
          Purity>95% immunoglobulin by SDS-PAGE
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          217669

          References

          Reference overview
          Kariv, I., et al. 1996. J. Immunol. 157, 29.
          61st Forum in Immunology. 1995. Res. Immunol. 146, 127.
          Jenkins, M.K., and Johnson, J.G. 1993. Curr. Opin. Immunol. 5, 361.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition

          Citations

          Title
        • Renee M. Marshall, et al. (2005) Cyclin T1 expression is regulated by multiple signaling pathways and mechanisms during activation of human peripheral blood lymphocytes. Journal of Immunology 175, 6402-6411.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision25-July-2007 RFH
          ApplicationFlow Cytometry (5 µg/ml)
          Frozen Sections (see comments)
          Immunoprecipitation (see comments)
          T cell Co-stimulation (see comments)
          DescriptionProtein A purified mouse monoclonal antibody. Recognizes the CD28 protein.
          BackgroundHuman CD28 is an important co-stimulatory molecule found on all CD4+ T cells and ~50% of CD8+ T cells. T cell activities attributed to CD28 include prevention of anergy, induction of cytokine gene transcription, stabilization of cytokine mRNA, and activation of CD8+ cytotoxic T lymphocytes. The ligands for CD28, known as CD80 (B7-1) and CD86 (B7-2), are monomeric transmembrane glycoproteins.
          HostMouse
          Immunogen speciesHuman
          Immunogenhuman CD28 Ig fusion protein and HPB-MLT human tumor cells
          CloneANC28.1/5D10
          IsotypeIgG₁
          Specieshuman
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Purity>95% immunoglobulin by SDS-PAGE
          Preservative500 µg/ml gentamycin sulfate
          CommentsMonomeric and cross-linked antibody will stimulate expression of IL-2 from CD28+ cells. FACS was performed on 5x105 cultured HPB-MLT cells using anti-CD28 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
          Toxicity Standard Handling
          ReferencesKariv, I., et al. 1996. J. Immunol. 157, 29.
          61st Forum in Immunology. 1995. Res. Immunol. 146, 127.
          Jenkins, M.K., and Johnson, J.G. 1993. Curr. Opin. Immunol. 5, 361.
          Citation
        • Renee M. Marshall, et al. (2005) Cyclin T1 expression is regulated by multiple signaling pathways and mechanisms during activation of human peripheral blood lymphocytes. Journal of Immunology 175, 6402-6411.