NE1004 Anti-Apolipoprotein E Mouse mAb (E6D7)

NE1004
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      NE1004-100UL
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      Stocked 
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      Available
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          Plastic ampoule 100 ul
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          Description
          OverviewRecognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum and brain.
          Catalogue NumberNE1004
          Brand Family Calbiochem®
          SynonymsAnti-ApoE
          Application Data
          Detection of human apolipoprotein E by immunoblotting. Sample: Human serum (1 μl). Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: chemiluminescence.

          Detection of human apolipoprotein E by staining paraffin sections. Sample: Vascular amyliod β Down's Syndrome brain tissue. Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: DAB.

          Detection of human apolipoprotein E by staining paraffin sections. Sample: Alzheimer's disease brain tissue containing senile plaques. Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: DAB
          References
          ReferencesGolabek, A.A., et al. 1996. J. Biol. Chem. 271, 10602.
          Product Information
          FormLiquid
          FormulationCulture supernatant diluted in PBS.
          Positive controlBrain tissue, serum
          PreservativeNone
          Applications
          Key Applications Immunoprecipitation
          Paraffin Sections
          Enzyme-Linked Immunosorbent Assay
          Frozen Sections
          Immunoblotting (Western Blotting)
          Application NotesELISA (see comments)
          Immunoblotting (1:100-1:1000)
          Immunoprecipitation (see comments)
          Frozen Sections (1:100-1:10,000)
          Paraffin Sections (1:100-1:10,000, formic acid or heat pre-treatment required)
          Application CommentsRecognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum, but multiple bands are seen when used with brain tissue samples. Staining of formalin-fixed paraffin-embedded tissues may be enhanced by the pre-treatment of tissue with 70% formic acid for 10-30 min at room temperature or by heat pre-treatment using citrate buffer, pH 6.0. This antibody has also been reported to work for immunoprecipitation and ELISA. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of ApoE
          ImmunogenHuman
          CloneE6D7
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Apolipoprotein E Mouse mAb (E6D7) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Apolipoprotein E Mouse mAb (E6D7) Certificates of Analysis

          TitleLot Number
          NE1004

          References

          Reference overview
          Golabek, A.A., et al. 1996. J. Biol. Chem. 271, 10602.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision07-October-2010 JSW
          SynonymsAnti-ApoE
          ApplicationELISA (see comments)
          Immunoblotting (1:100-1:1000)
          Immunoprecipitation (see comments)
          Frozen Sections (1:100-1:10,000)
          Paraffin Sections (1:100-1:10,000, formic acid or heat pre-treatment required)
          Application Data
          Detection of human apolipoprotein E by immunoblotting. Sample: Human serum (1 μl). Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: chemiluminescence.

          Detection of human apolipoprotein E by staining paraffin sections. Sample: Vascular amyliod β Down's Syndrome brain tissue. Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: DAB.

          Detection of human apolipoprotein E by staining paraffin sections. Sample: Alzheimer's disease brain tissue containing senile plaques. Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: DAB
          DescriptionMouse monoclonal antibody supplied as diluted culture supernatant. Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E.
          BackgroundApolipoprotein E (Apo E) is a polymorphic, multifunctional protein synthesized by liver, kidney, brain, and other cell types. It has been reported that the genetic inheritance of apolipoprotein isoform E4 is a significant risk factor for late onset Alzheimer's Disease (AD).
          HostMouse
          Immunogen speciesHuman
          Immunogena synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of ApoE
          CloneE6D7
          IsotypeIgG₁
          Specieshuman, not mouse, not rabbit
          Positive controlBrain tissue, serum
          FormLiquid
          FormulationCulture supernatant diluted in PBS.
          Concentration Label Please refer to vial label for lot-specific concentration
          PreservativeNone
          CommentsRecognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum, but multiple bands are seen when used with brain tissue samples. Staining of formalin-fixed paraffin-embedded tissues may be enhanced by the pre-treatment of tissue with 70% formic acid for 10-30 min at room temperature or by heat pre-treatment using citrate buffer, pH 6.0. This antibody has also been reported to work for immunoprecipitation and ELISA. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesGolabek, A.A., et al. 1996. J. Biol. Chem. 271, 10602.