MABS449 Anti-Acylation Stimulating Protein Antibody, clone 4H3

MABS449
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ELISA M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABS449
      DescriptionAnti-Acylation Stimulating Protein Antibody, clone 4H3
      Alternate Names
      • Complement C3
      • C3adesArg
      • C3
      • PZP-like alpha-2-macroglobulin domain-containing protein 1
      Background InformationThe protein called Acylation stimulating protein (ASP) is a cleavage fragment protein from the Complement C3 gene. C3 plays a central role in the activation of the complement system. C3’s processing by C3 convertase is the central reaction in both classical and alternative complement pathways. ASP acts as an adipogenic hormone that stimulates triglyceride synthesis and glucose transport in adipocytes, and thus it helps regulate fat storage and triglyceride synthesis. ASP stimulates triglyceride synthesis via PLC, MAPK and AKT signaling pathways and ASP promotes the phosphorylation and internalization and recycling of Complement factor C5AR2. ASP is expressed in adipocytes and released into the plasma during both the fasting and postprandial periods. Increased levels of C3 and its cleavage product ASP are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Acylation Stimulating Protein Antibody, clone 4H3 is validated for use in Western Blotting and ELISA for the detection of Acylation Stimulating Protein.
      Key Applications
      • Western Blotting
      • ELISA
      Application NotesELISA Analysis: 7 µg/mL of this antibody detected ASP (PEG precipitated) in a sandwich ELISA format (Saleh, J,. et al, J. Lipid Res. 1998. 39: 884–891)
      Biological Information
      Immunogenlinear peptide corresponding to Human Acylation Stimulating Protein.
      Clone4H3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CPAMD1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~ 16/10 kDa observed, 10 kDa indicates native ASP, 16 kDa indicates HIS-Tag ASP. HIS-Tag was added for purification purposes. We have found no difference in Activity (measured by TGS, Fatty Acid uptake, etc) between rASP with/without the tag. Further, the ELISA was successful in measuring both.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting on purified Acylation Stimulating Protein from transfected HEK293 cell lysate

      Western Blotting Analysis: 0.1 µg/mL of this antibody detected purified Acylation Stimulating Protein from transfected HEK293 cell.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Acylation Stimulating Protein Antibody, clone 4H3 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Acylation Stimulating Protein Antibody, clone 4H3 Certificates of Analysis

      TitleLot Number
      Anti-Acylation Stimulating Protein, clone 4H3Q2476319

      References

      Reference overviewPub Med ID
      Coordinated release of acylation stimulating protein (ASP) and triacylglycerol clearance by human adipose tissue in vivo in the postprandial period.
      Saleh, J; Summers, LK; Cianflone, K; Fielding, BA; Sniderman, AD; Frayn, KN
      Journal of lipid research  39  884-91  1998

      Show Abstract
      9555951 9555951

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies