ABS1020 Anti-Acylation Stimulating Protein Antibody

ABS1020
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, FC, NEUT, ELISA Rb Purified Polyclonal Antibody
      Description
      Catalogue NumberABS1020
      DescriptionAnti-Acylation Stimulating Protein Antibody
      Alternate Names
      • Complement C3
      • C3adesArg
      • C3
      • PZP-like alpha-2-macroglobulin domain-containing protein 1
      Background InformationThe protein called Acylation stimulating protein (ASP) is a cleavage fragment protein from the Complement C3 gene. C3 plays a central role in the activation of the complement system. C3’s processing by C3 convertase is the central reaction in both classical and alternative complement pathways. ASP acts as an adipogenic hormone that stimulates triglyceride synthesis and glucose transport in adipocytes, and thus it helps regulate fat storage and triglyceride synthesis. ASP stimulates triglyceride synthesis via PLC, MAPK and AKT signaling pathways and ASP promotes the phosphorylation and internalization and recycling of Complement factor C5AR2. ASP is expressed in adipocytes and released into the plasma during both the fasting and postprandial periods. Increased levels of C3 and its cleavage product ASP are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit Polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Acylation Stimulating Protein Antibody is validated for use in Western Blotting and Flow Cytometry and Neutralizing and ELISA for the detection of Acylation Stimulating Protein.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Neutralizing
      • ELISA
      Application NotesFlow Cytometry Analysis: 10 µg/mL of this antibody blocked ASP binding to HEK-hCL52 cells (Wei, C,. et al, Am J Physiol Endocrinol Metab 293:E1482-E1491, 2007)
      Neutralizing Assay Analysis: A representative lot of this antibody demonstrated neutralizing effect of ASP stimulation of TG synthesis and glucose transport in HEK-hC5L2 cells,
      3T3-L1 preadipocytes, and 3T3-L1 adipocytes (Wei, C,. et al, Am J Physiol Endocrinol Metab 293:E1482-E1491, 2007)
      ELISA Analysis: 0.5 µg/mL of this antibody detected ASP (PEG precipitated) in a sandwich ELISA format (Saleh, J,. et al, J. Lipid Res. 1998. 39: 884–891)
      Biological Information
      Immunogenrecombinant protein corresponding to Human Acylation Stimulating Protein.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CPAMD1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~ 16/10 kDa observed, 10 kDa indicates native ASP, 16 kDa indicates HIS-Tag ASP. HIS-Tag was added for purification purposes. We have found no difference in Activity (measured by TGS, Fatty Acid uptake, etc) between rASP with/without the tag. Further, the ELISA was successful in measuring both.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 expressing ASP cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Acylation Stimulating Protein in 10 µg of HEK293 expressing ASP cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Acylation Stimulating Protein Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Acylation Stimulating Protein Antibody Certificates of Analysis

      TitleLot Number
      Anti-Acylation Stimulating ProteinQ2473133

      References

      Reference overviewPub Med ID
      The effects of acylation stimulating protein supplementation VS antibody neutralization on energy expenditure in wildtype mice.
      Paglialunga, S; Fisette, A; Munkonda, M; Gao, Y; Richard, D; Cianflone, K
      BMC physiology  10  4  2010

      Show Abstract
      20416070 20416070
      Acylation-stimulating protein/C5L2-neutralizing antibodies alter triglyceride metabolism in vitro and in vivo.
      Cui, W; Paglialunga, S; Kalant, D; Lu, H; Roy, C; Laplante, M; Deshaies, Y; Cianflone, K
      American journal of physiology. Endocrinology and metabolism  293  E1482-91  2007

      Show Abstract
      17711993 17711993
      Coordinated release of acylation stimulating protein (ASP) and triacylglycerol clearance by human adipose tissue in vivo in the postprandial period.
      Saleh, J; Summers, LK; Cianflone, K; Fielding, BA; Sniderman, AD; Frayn, KN
      Journal of lipid research  39  884-91  1998

      Show Abstract
      9555951 9555951

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies