MAB1501R Anti-Actin Antibody,clone C4

MAB1501R
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      A ELISA, ICC, IHC, IH(P), WB M Protein G Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB1501R
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Actin Antibody,clone C4
      Alternate Names
      • actin, alpha 1, skeletal muscle
      • alpha skeletal muscle actin
      • MAB1501
      • MAB1501X
      • smooth muscle actin
      • SMA
      Background InformationActins are ubiquitous eukaryotic proteins that serve as multi-functional, basic building blocks of cytoskeletal microfilaments. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, transcriptional regulation, and vesicle trafficking. These functions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues.
      Beta- and gamma-actins, also known as cytoplasmic actins, are highly conserved in higher animals and are predominantly expressed in non-muscle cells where they control cell structure and motility. They are nearly identical proteins and differ only in four amino acids at the N-terminal region. The other four actin isoforms are typically found in specific adult muscle tissue types. alpha-cardiac and alpha-skeletal actins are expressed in striated cardiac and skeletal muscles, respectively. Alpha and gamma actins are primarily found in vascular smooth muscle and enteric smooth muscles, respectively. It has been shown that under calcium-bound conditions, beta-actin exhibits more dynamic behavior than gamma-actin with higher rates of polymerization and depolymerization. Also, beta- and gamma-actins can readily copolymerize, and the resulting filaments exhibit polymerization and depolymerization rates that vary depending on the ratio of beta- to gamma-actin (Lessard, JL., et al (1988). Cell Motility Cytoskeleton 10(3); 349-362).
      References
      Product Information
      FormatProtein G Purified
      Control
      • HeLa whole cell lysate.
      PresentationProtein G Purified in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% NaN3.
      Applications
      ApplicationThis Anti-Actin Antibody, clone C4 is validated for use in ELISA, IC, IH, IH(P), WB for the detection of Actin and has published in over 70 citations.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunocytochemistry:
      10 μg/mL dilution from a previous lot was used (methanol fixed mouse 3T3 cells).

      Immunohistochemistry:
      10μg/mL dilution from a previous lot was used for paraffin embedded, 4% formaldehyde, 3% glutaraldehyde, sodium cacodylate treated sections {see Luciano, L et al. 2003}.

      ELISA:
      A previous lot was shown to be strongly reactive with the cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum.

      Western blot:
      1-20 µg/ml. On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43 kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha-actin found in skeletal, cardiac, and arterial muscle (Otey, 1987).

      Immunohistochemistry:
      10µg/mL for paraffin embedded, 4% formaldehyde, 3% glutaraldehyde, sodium cacodylate treated sections {see Luciano, L et al. 2003}.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified chicken gizzard actin (Lessard, 1988).
      EpitopeEpitope is conserved in all known actins.
      CloneC4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityMAB1501R is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertabrate actin (Lessard, 1988). Reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts. Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988).
      IsotypeIgG1κ
      Species Reactivity
      • All
      Species Reactivity NoteTo date, all animal species and cell types with an actin form react by indirect immunofluorescence or immunoblot, including plant actin.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
      Gene Symbol
      • ACTA1
      • MPFD
      • ASMA
      • NEM1
      • CFTDM
      • ACTA
      • CFTD
      • NEM2
      • Alpha-actin-1
      • CFTD1
      • NEM3
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P68133 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
      SIZE: 377 amino acids; 42051 Da
      SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID.
      SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
      DISEASE: SwissProt: P68133 # Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. Nemaline myopathy (NEM) is a form of congenital myopathy characterized by abnormal thread- or rod-like structures in muscle fibers on histologic examination. The clinical phenotype is highly variable, with differing age at onset and severity. & Defects in ACTA1 are a cause of congenital myopathy with excess of thin myofilaments (CM) [MIM:102610]. & Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
      SIMILARITY: SwissProt: P68133 ## Belongs to the actin family.
      MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
      Molecular Weight43 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HeLa lysate.

      Western Blot Analysis:
      1:1000 dilution of this lot detected actin on 10 μg of HeLa lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Actin Antibody,clone C4 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Actin Antibody,clone C4 Certificates of Analysis

      TitleLot Number
      Anti-Actin, Clone C4 - 21421712142171
      Anti-Actin, Clone C4 - 23878162387816
      Anti-Actin, Clone C4 - 23910732391073
      Anti-Actin, Clone C4 - 24596312459631
      Anti-Actin, Clone C4 - 19912581991258
      Anti-Actin, Clone C4 - 20202802020280
      Anti-Actin, Clone C4 - 20660012066001
      Anti-Actin, Clone C4 - 21884932188493
      Anti-Actin, Clone C4 - 22795362279536
      Anti-Actin, Clone C4 - 24890212489021

      References

      Reference overviewApplicationSpeciesPub Med ID
      Roles of PCNA ubiquitination and TLS polymerases κ and η in the bypass of methyl methanesulfonate-induced DNA damage.
      Wit, N; Buoninfante, OA; van den Berk, PC; Jansen, JG; Hogenbirk, MA; de Wind, N; Jacobs, H
      Nucleic acids research  43  282-94  2015

      Show Abstract
      25505145 25505145
      Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells.
      Eino, A; Kageyama, S; Uemura, T; Annoh, H; Saito, T; Narita, I; Waguri, S; Komatsu, M
      Journal of cell science  128  4453-61  2015

      Show Abstract
      26483381 26483381
      Hypoxia-induced gene expression results from selective mRNA partitioning to the endoplasmic reticulum.
      Staudacher, JJ; Naarmann-de Vries, IS; Ujvari, SJ; Klinger, B; Kasim, M; Benko, E; Ostareck-Lederer, A; Ostareck, DH; Bondke Persson, A; Lorenzen, S; Meier, JC; Blüthgen, N; Persson, PB; Henrion-Caude, A; Mrowka, R; Fähling, M
      Nucleic acids research  43  3219-36  2015

      Show Abstract
      Western Blotting25753659 25753659
      Wilms' tumor gene 1 regulates p63 and promotes cell proliferation in squamous cell carcinoma of the head and neck.
      Li, X; Ottosson, S; Wang, S; Jernberg, E; Boldrup, L; Gu, X; Nylander, K; Li, A
      BMC cancer  15  342  2015

      Show Abstract
      25929687 25929687
      Human Cytomegalovirus miR-UL112-3p Targets TLR2 and Modulates the TLR2/IRAK1/NFκB Signaling Pathway.
      Landais, I; Pelton, C; Streblow, D; DeFilippis, V; McWeeney, S; Nelson, JA
      PLoS pathogens  11  e1004881  2015

      Show Abstract
      25955717 25955717
      DOT1L cooperates with the c-Myc-p300 complex to epigenetically derepress CDH1 transcription factors in breast cancer progression.
      Cho, MH; Park, JH; Choi, HJ; Park, MK; Won, HY; Park, YJ; Lee, CH; Oh, SH; Song, YS; Kim, HS; Oh, YH; Lee, JY; Kong, G
      Nature communications  6  7821  2015

      Show Abstract
      26199140 26199140
      Sirt1-deficiency causes defective protein quality control.
      Tomita, T; Hamazaki, J; Hirayama, S; McBurney, MW; Yashiroda, H; Murata, S
      Scientific reports  5  12613  2015

      Show Abstract
      26219988 26219988
      Redundant Roles of Rpn10 and Rpn13 in Recognition of Ubiquitinated Proteins and Cellular Homeostasis.
      Hamazaki, J; Hirayama, S; Murata, S
      PLoS genetics  11  e1005401  2015

      Show Abstract
      26222436 26222436
      The unexpected role of polyubiquitin chains in the formation of fibrillar aggregates.
      Morimoto, D; Walinda, E; Fukada, H; Sou, YS; Kageyama, S; Hoshino, M; Fujii, T; Tsuchiya, H; Saeki, Y; Arita, K; Ariyoshi, M; Tochio, H; Iwai, K; Namba, K; Komatsu, M; Tanaka, K; Shirakawa, M
      Nature communications  6  6116  2015

      Show Abstract
      25600778 25600778
      Gq signaling causes glomerular injury by activating TRPC6.
      Wang, L; Jirka, G; Rosenberg, PB; Buckley, AF; Gomez, JA; Fields, TA; Winn, MP; Spurney, RF
      The Journal of clinical investigation  125  1913-26  2015

      Show Abstract
      25844902 25844902

      Data Sheet

      Title
      Anti-Actin, near a.a. 50-70, clone C4 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies