MAB1501 Anti-Actin Antibody, clone C4

MAB1501
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      A ELISA, ICC, IF, IHC, IH(P), WB M Ascites Monoclonal Antibody
      Description
      Catalogue NumberMAB1501
      Replaces04-1040
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Actin Antibody, clone C4
      Alternate Names
      • MAB1501X
      • MAB1501R
      Background InformationActins are ubiquitous eukaryotic proteins that serve as multi-functional, basic building blocks of cytoskeletal microfilaments. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, transcriptional regulation, and vesicle trafficking. These functions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues.
      Beta- and gamma-actins, also known as cytoplasmic actins, are highly conserved in higher animals and are predominantly expressed in non-muscle cells where they control cell structure and motility. They are nearly identical proteins and differ only in four amino acids at the N-terminal region. The other four actin isoforms are typically found in specific adult muscle tissue types. alpha-cardiac and alpha-skeletal actins are expressed in striated cardiac and skeletal muscles, respectively. Alpha and gamma actins are primarily found in vascular smooth muscle and enteric smooth muscles, respectively. It has been shown that under calcium-bound conditions, beta-actin exhibits more dynamic behavior than gamma-actin with higher rates of polymerization and depolymerization. Also, beta- and gamma-actins can readily copolymerize, and the resulting filaments exhibit polymerization and depolymerization rates that vary depending on the ratio of beta- to gamma-actin (Lessard, JL., et al (1988). Cell Motility Cytoskeleton 10(3); 349-362).
      References
      Product Information
      FormatAscites
      Control
      • HeLa whole cell lysate.
      PresentationMouse monoclonal Ascites fluid, with 0.01% sodium azide.
      Applications
      ApplicationReliably and specifically detect actin using this Anti-Actin Antibody, clone C4. This highly published monoclonal antibody is validated for use in ELISA, IC, IF, IH, IH(P) & WB. This mAb is also available as a fluorescent conjugate.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesIndirect immunofluorescence at 1:100:
      Tissue culture cells -- fix with formaldehyde, treat with methanol or acetone.
      Glycerinated myofibrils -- fix fibers with formaldehyde, treat with cold methanol. Stains I-bands intensely and stress fibers in human fibroblasts.
      Cryostat sections (6 µm) -- quick frozen in isopentane, slides treated with gelatin and formaldehyde.

      Immunoblots:
      1:100-1:1,000 (Otey, 1987):On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43 kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha-actin found in skeletal, cardiac, and arterial muscle.

      Iodination (Lessard, 1979).

      Solid phase binding assay ELISA:
      1:800-1:1,000 dilution from a previous lot was shown to be strongly reactive with cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum.

      ELISA:
      strongly reactive with the cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified chicken gizzard actin (Lessard, 1988).
      EpitopeEpitope is conserved in all known actins.
      CloneC4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityMAB1501 is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertabrate actin (Lessard, 1988). Reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts. Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988).
      IsotypeIgG1κ
      Species Reactivity
      • All
      Species Reactivity NoteTo date, all animal species and cell types with an actin form react by indirect immunofluorescence or immunoblot, including plant actin.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
      Gene Symbol
      • ACTA1
      • MPFD
      • ASMA
      • NEM1
      • CFTDM
      • ACTA
      • CFTD
      • NEM2
      • Alpha-actin-1
      • CFTD1
      • NEM3
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P68133 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
      SIZE: 377 amino acids; 42051 Da
      SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID.
      SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
      DISEASE: SwissProt: P68133 # Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. Nemaline myopathy (NEM) is a form of congenital myopathy characterized by abnormal thread- or rod-like structures in muscle fibers on histologic examination. The clinical phenotype is highly variable, with differing age at onset and severity. & Defects in ACTA1 are a cause of congenital myopathy with excess of thin myofilaments (CM) [MIM:102610]. & Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
      SIMILARITY: SwissProt: P68133 ## Belongs to the actin family.
      MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
      Molecular Weight43 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on A431 lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected ACTIN on 10 ug of A431 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at -20°C in undiluted aliquots for up to 12 months from date of receipt. Do not store in a diluted format. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Actin Antibody, clone C4 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Actin Antibody, clone C4 Certificates of Analysis

      TitleLot Number
      Anti-Actin, Clone C4 - 32613023261302
      Anti-Actin, Clone C4 - 32825323282532
      Anti-Actin, clone C43166064
      Anti-Actin, clone C42464499
      Anti-Actin, clone C4 -26914302691430
      Anti-Actin, clone C4 - 21459282145928
      Anti-Actin, clone C4 - 23846412384641
      Anti-Actin, clone C4 - 19943581994358
      Anti-Actin, clone C4 - 20204862020486
      Anti-Actin, clone C4 - 20659872065987

      References

      Reference overviewApplicationSpeciesPub Med ID
      Increased Infiltration of Extra-Cardiac Cells in Myxomatous Valve Disease.
      Sauls, K; Toomer, K; Williams, K; Johnson, AJ; Markwald, RR; Hajdu, Z; Norris, RA
      Journal of cardiovascular development and disease  2  200-213  2015

      Show Abstract
      26473162 26473162
      Let-7-mediated suppression of mucin 1 expression in the mouse uterus during embryo implantation.
      Inyawilert, W; Fu, TY; Lin, CT; Tang, PC
      The Journal of reproduction and development  61  138-44  2015

      Show Abstract
      25739861 25739861
      AMPA Receptor-mTOR Activation is Required for the Antidepressant-Like Effects of Sarcosine during the Forced Swim Test in Rats: Insertion of AMPA Receptor may Play a Role.
      Chen, KT; Tsai, MH; Wu, CH; Jou, MJ; Wei, IH; Huang, CC
      Frontiers in behavioral neuroscience  9  162  2015

      Show Abstract
      26150775 26150775
      The ribonucleotidyl transferase USIP-1 acts with SART3 to promote U6 snRNA recycling.
      Rüegger, S; Miki, TS; Hess, D; Großhans, H
      Nucleic acids research  43  3344-57  2015

      Show Abstract
      25753661 25753661
      Autophagy Regulates Formation of Primary Cilia in Mefloquine-Treated Cells.
      Shin, JH; Bae, DJ; Kim, ES; Kim, HB; Park, SJ; Jo, YK; Jo, DS; Jo, DG; Kim, SY; Cho, DH
      Biomolecules & therapeutics  23  327-32  2015

      Show Abstract
      26157548 26157548
      Unique Effects of Acute Aripiprazole Treatment on the Dopamine D2 Receptor Downstream cAMP-PKA and Akt-GSK3β Signalling Pathways in Rats.
      Pan, B; Chen, J; Lian, J; Huang, XF; Deng, C
      PloS one  10  e0132722  2015

      Show Abstract
      26162083 26162083
      TORC1 promotes phosphorylation of ribosomal protein S6 via the AGC kinase Ypk3 in Saccharomyces cerevisiae.
      González, A; Shimobayashi, M; Eisenberg, T; Merle, DA; Pendl, T; Hall, MN; Moustafa, T
      PloS one  10  e0120250  2015

      Show Abstract
      25767889 25767889
      STAT3 upregulation in pituitary somatotroph adenomas induces growth hormone hypersecretion.
      Zhou, C; Jiao, Y; Wang, R; Ren, SG; Wawrowsky, K; Melmed, S
      The Journal of clinical investigation  125  1692-702  2015

      Show Abstract
      25774503 25774503
      Recognition of Linear B-Cell Epitope of Betanodavirus Coat Protein by RG-M18 Neutralizing mAB Inhibits Giant Grouper Nervous Necrosis Virus (GGNNV) Infection.
      Chen, CW; Wu, MS; Huang, YJ; Cheng, CA; Chang, CY
      PloS one  10  e0126121  2015

      Show Abstract
      25938761 25938761
      Prdx4 is a compartment-specific H2O2 sensor that regulates neurogenesis by controlling surface expression of GDE2.
      Yan, Y; Wladyka, C; Fujii, J; Sockanathan, S
      Nature communications  6  7006  2015

      Show Abstract
      25943695 25943695

      Brochure

      Title
      AXIS: Axon Investigation System
      SNAP i.d. 2.0 System Brochure

      Technical Info

      Title
      Protein Blotting Handbook: 6th Edition (MilliporeSigma)

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies