NA02A Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate

NA02A
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      NA02A-0.5ML
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          Plastic ampoule 0.5 ml
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          Description
          OverviewRecognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs). Also recognizes 7-methylguanosine-capped mRNA.
          Catalogue NumberNA02A
          Brand Family Calbiochem®
          References
          ReferencesCasciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
          Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
          Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71.
          Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
          Krainer, A.R. and Maniatis, T. 1985. Cell 42, 725.
          Kramer, A., et al. 1984. Cell 38, 299.
          Lerner, M.R., et al. 1980. Nature 283, 220.
          Product Information
          FormLiquid suspension
          FormulationIn PBS.
          Negative controlTransfer RNA (tRNA)
          Positive controlHeterogenous nuclear RNA (hnRNA)
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesAffinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599.
          Key Applications Affinity Purification
          Immunoblotting (Western Blotting)
          Application NotesAffinity Purification (see application references)
          Immunoprecipitation (15 µl beads/sample)
          Immunoprecipitation (1-2 µg/ml; see application references)
          Immunocytochemistry (use Cat. No. NA02)
          Paraffin Sections (use Cat. No. NA02)
          Application CommentsAlso recognizes 7-methylguanosine-capped mRNA. 2,2,7-trimethylguanosine (Ab-1) is human, mouse, and rat reactive. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography. All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual sample types.
          Biological Information
          Immunogen2,2,7-trimethylguanosine
          CloneK121
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate Certificates of Analysis

          TitleLot Number
          NA02A

          References

          Reference overview
          Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
          Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
          Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71.
          Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
          Krainer, A.R. and Maniatis, T. 1985. Cell 42, 725.
          Kramer, A., et al. 1984. Cell 38, 299.
          Lerner, M.R., et al. 1980. Nature 283, 220.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision04-September-2007 RFH
          ApplicationAffinity Purification (see application references)
          Immunoprecipitation (15 µl beads/sample)
          Immunoprecipitation (1-2 µg/ml; see application references)
          Immunocytochemistry (use Cat. No. NA02)
          Paraffin Sections (use Cat. No. NA02)
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Conjugated to agarose. Recognizes the exposed 5' cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).
          BackgroundThe U small nuclear ribonucleoprotein particles (snRNPs) are present in all eukaryotes studied thus far and are involved in the splicing of RNA molecules. snRNPs are composed of several proteins and one or two RNA molecules. The RNA molecules (snRNAs) contain a unique 5' terminal cap structure containing the nucleoside 2,2,7-trimethylguanosine. The 70 kDa protein component of U1 snRNP is cleaved during apoptosis, perhaps by a caspase. Monoclonal antibodies to the 5' cap structure react only to snRNAs.
          HostMouse
          Immunogen2,2,7-trimethylguanosine
          CloneK121
          IsotypeIgG₁
          Specieshuman, mouse, rat
          Positive controlHeterogenous nuclear RNA (hnRNA)
          Negative controlTransfer RNA (tRNA)
          FormLiquid suspension
          FormulationIn PBS.
          Preservative≤0.1% sodium azide
          CommentsAlso recognizes 7-methylguanosine-capped mRNA. 2,2,7-trimethylguanosine (Ab-1) is human, mouse, and rat reactive. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography. All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual sample types.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesCasciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
          Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
          Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71.
          Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
          Krainer, A.R. and Maniatis, T. 1985. Cell 42, 725.
          Kramer, A., et al. 1984. Cell 38, 299.
          Lerner, M.R., et al. 1980. Nature 283, 220.
          Application referencesAffinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599.

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          Categories

          Life Science Research > Antibodies and Assays > Primary Antibodies