NA02 Anti-2,2,7-Trimethylguanosine Mouse mAb (K121)

NA02
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      NA02-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).
          Catalogue NumberNA02
          Brand Family Calbiochem®
          Application Data
          Detection of the 5' cap of snRNA by immunocytochemistry. Sample: HeLa cells fixed with methanol. Primary antibody: Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) (Cat. No. NA02) (0.8 μg/ml). Detection: fluorescence.
          References
          ReferencesCasciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
          Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
          Reddy, R., and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
          Krainer, A.R., and Maniatis, T. 1985. Cell 42, 725.
          Kramer, A., et al. 1984. Cell 38, 299.
          Lerner, M.R., et al. 1980. Nature 283, 220.
          Product Information
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Negative controlTransfer RNA (tRNA)
          Positive controlHeterogenous nuclear RNA (hnRNA)
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesAffinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599. Paraffin Sections Volm, M., et al. 2000. Clin. Cancer Res. 6, 3236.
          Key Applications Immunocytochemistry
          Immunoprecipitation
          Paraffin Sections
          Application NotesAffinity Purification (use Cat. No. NA02A, see application references)
          Immunocytochemistry (0.5-5 µg/ml)
          Immunoprecipitation (1-2 µg, see application references)
          Paraffin Sections (see application references)
          Application CommentsAlso recognizes 7-methylguanosine-capped mRNA. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography (use Cat. No. NA02A). All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogen2,2,7-trimethylguanosine
          CloneK121
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Certificates of Analysis

          TitleLot Number
          NA02

          References

          Reference overview
          Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
          Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
          Reddy, R., and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
          Krainer, A.R., and Maniatis, T. 1985. Cell 42, 725.
          Kramer, A., et al. 1984. Cell 38, 299.
          Lerner, M.R., et al. 1980. Nature 283, 220.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision04-September-2007 RFH
          ApplicationAffinity Purification (use Cat. No. NA02A, see application references)
          Immunocytochemistry (0.5-5 µg/ml)
          Immunoprecipitation (1-2 µg, see application references)
          Paraffin Sections (see application references)
          Application Data
          Detection of the 5' cap of snRNA by immunocytochemistry. Sample: HeLa cells fixed with methanol. Primary antibody: Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) (Cat. No. NA02) (0.8 μg/ml). Detection: fluorescence.
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).
          BackgroundThe U small nuclear ribonucleoprotein particles (snRNPs) are present in all eukaryotes studied thus far and are involved in the splicing of RNA molecules. snRNPs are composed of several proteins and one or two RNA molecules. The RNA molecules (snRNAs) contain a unique 5' terminal cap structure containing the nucleoside 2,2,7-trimethylguanosine. The 70 kDa protein component of U1 snRNP is cleaved during apoptosis, perhaps by a caspase. Monoclonal antibodies to the 5' cap structure react only to snRNAs.
          HostMouse
          Immunogen2,2,7-trimethylguanosine
          CloneK121
          IsotypeIgG₁
          Specieshuman, mouse, rat
          Positive controlHeterogenous nuclear RNA (hnRNA)
          Negative controlTransfer RNA (tRNA)
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsAlso recognizes 7-methylguanosine-capped mRNA. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography (use Cat. No. NA02A). All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesCasciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
          Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
          Reddy, R., and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
          Krainer, A.R., and Maniatis, T. 1985. Cell 42, 725.
          Kramer, A., et al. 1984. Cell 38, 299.
          Lerner, M.R., et al. 1980. Nature 283, 220.
          Application referencesAffinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599. Paraffin Sections Volm, M., et al. 2000. Clin. Cancer Res. 6, 3236.

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          Categories

          Life Science Research > Antibodies and Assays > Primary Antibodies