SCR004 Alkaline Phosphatase Detection Kit

SCR004
100 assays  
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      Cult
      Description
      Catalogue NumberSCR004
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAlkaline Phosphatase Detection Kit
      OverviewMillipore's Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.
      Materials Required but Not Delivered1. Fixative (e.g. 4% Paraformaldehyde in PBS)

      2. 1 x Rinse Buffer (e.g. TBST: 20mM Tris-HCl, pH 7.4, 0.15M NaCl, 0.05% Tween-20)

      3. Hematoxylin (optional)

      4. Microscope
      References
      Product Information
      Components
      • Fast Red Violet solution (0.8g/L stock) (Part No. 90239). Two 15mL bottles.
      • Napthol AS-BI phosphate solution (4mg/mL) in AMPD buffer (2mol/L), pH 9.5 (Part No. 90234). One 15mL bottle.
      Applications
      ApplicationThis Alkaline Phosphatase Detection Kit is a specific & sensitive tool for the phenotypic assessment of Embryonic Stem (ES) cell differentiation by the determination of AP activity.
      Key Applications
      • Stem Cell Culture
      Application NotesEmbryonic stem (ES) cells are totipotent cells derived from the inner cell mass (ICM) of preimplantation mammalian embryos and are capable of unlimited, undifferentiated proliferation in vitro [1,2,3]. Undifferentiated murine ES cells can be maintained in vitro for extended periods in media containing the cytokine, leukemia-inhibitory factor (LIF) or Millipore's proprietary ES cell culture reagent, ESGRO [4,5]. The undifferentiated state of ES cells can be characterized by high level of expression of Alkaline Phosphatase (AP) [6], the expression of surface markers including SSEA and TRA antigens and the transcription factor Oct-4.

      Millipore's Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.

      Available separately from Millipore are the monoclonal antibodies TRA-2-49 (Catalog Number MAB4349) and TRA-2-54 (Catalog Number MAB4354), which permit the detection of Liver / Bone / Kidney isozyme of AP as expressed by ES cells. When used in flow cytometry, these reagents permit a quantitative assessment of AP expression by ES cells [7].

      For Research Use Only; Not for use in diagnostic procedures
      Biological Information
      Species Reactivity
      • Mammals
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      • Human Embryonic Stem Cells
      • Mesenchymal Stem Cells
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      • Epithelial Cells
      • Pancreatic Stem Cells
      • Cardiac Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe Alkaline Phosphatase Detection Kit consists of two components used for determining AP activity.

      When stored at 2-8º C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.
      Packaging Information
      Material Size100 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewApplicationPub Med ID
      Efficient iPS cell production with the MyoD transactivation domain in serum-free culture.
      Hiroyuki Hirai,Nobuko Katoku-Kikyo,Peter Karian,Meri Firpo,Nobuaki Kikyo
      PloS one  7  2012

      Show Abstract
      22479546 22479546
      Establishment of LIF-dependent human iPS cells closely related to basic FGF-dependent authentic iPS cells.
      Hiroyuki Hirai,Meri Firpo,Nobuaki Kikyo
      PloS one  7  2012

      Show Abstract
      Immunofluorescence22720020 22720020
      BMP4 Signaling Acts via dual-specificity phosphatase 9 to control ERK activity in mouse embryonic stem cells.
      Zhongwei Li,Teng Fei,Jianping Zhang,Gaoyang Zhu,Lu Wang,Danyu Lu,Xiaochun Chi,Yan Teng,Ning Hou,Xiao Yang,Hongquan Zhang,Jing-Dong J Han,Ye-Guang Chen
      Cell stem cell  10  2012

      Show Abstract
      22305567 22305567
      Efficient feeder-free episomal reprogramming with small molecules.
      Junying Yu,Kevin Fongching Chau,Maxim A Vodyanik,Jinlan Jiang,Yong Jiang
      PloS one  6  2011

      Show Abstract Full Text Article
      21390254 21390254
      Large scale phosphoproteome profiles comprehensive features of mouse embryonic stem cells.
      Li, QR; Xing, XB; Chen, TT; Li, RX; Dai, J; Sheng, QH; Xin, SM; Zhu, LL; Jin, Y; Pei, G; Kang, JH; Li, YX; Zeng, R
      Molecular & cellular proteomics : MCP  10  M110.001750  2011

      Show Abstract
      21149613 21149613
      Ethanol, acetaldehyde, and estradiol affect growth and differentiation of rhesus monkey embryonic stem cells.
      Catherine A VandeVoort,Dana L Hill,Charles L Chaffin,Alan J Conley
      Alcoholism, clinical and experimental research  35  2011

      Show Abstract
      21438889 21438889
      Establishment of induced pluripotent stem cells from aged mice using bone marrow-derived myeloid cells.
      Zhao Cheng,Sachiko Ito,Naomi Nishio,Hengyi Xiao,Rong Zhang,Haruhiko Suzuki,Yayoi Okawa,Toyoaki Murohara,Ken-Ichi Isobe
      Journal of molecular cell biology  3  2011

      Show Abstract
      21228011 21228011
      Epigenetic regulation of Nanog expression by Ezh2 in pluripotent stem cells.
      Villasante, A; Piazzolla, D; Li, H; Gomez-Lopez, G; Djabali, M; Serrano, M
      Cell cycle (Georgetown, Tex.)  10  1488-98  2011

      Show Abstract
      Immunoprecipitation21490431 21490431
      Embryonic stem cells require Wnt proteins to prevent differentiation to epiblast stem cells.
      Derk ten Berge,Dorota Kurek,Tim Blauwkamp,Wouter Koole,Alex Maas,Elif Eroglu,Ronald K Siu,Roel Nusse
      Nature cell biology  13  2011

      Show Abstract
      21841791 21841791
      Radical acceleration of nuclear reprogramming by chromatin remodeling with the transactivation domain of MyoD.
      Hirai, Hiroyuki, et al.
      Stem Cells, 29: 1349-61 (2011)  2011

      Show Abstract
      21732495 21732495

      Brochure

      Title
      Human Stem Cell Systems
      Murine Embryonic Stem Cell Culture

      Technical Info

      Title
      A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions
      Accumax Dissociation Reagent for the Serial Passaging of Human Embryonic Stem Cells
      Collagenase Type I for Enzymatic Passaging of Human Embryonic Stem Cells in HEScGRO Medium
      Millipore Tools for Characterizing Induced Pluripotent Stem Cells
      OsteoMAX-XF™: A Novel Serum-free and Xeno-free Differentiation Medium for Accelerated Osteogenic Differentiation of Human Mesenchymal Stem Cells
      Single transfection of a synthetic polycistronic self-replicative RNA yields high numbers of transgene-free human iPSCs

      Data Sheet

      Title
      STEMCCA Lentivirus Reprogramming Kits

      User Guides

      Title
      Alkaline Phosphatase Detection Kit