L1613-BC 1 M HEPES Buffer (50x)

L1613-BC
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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      L1613
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          100 ml
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          Description
          OverviewHEPES (hydroxyethyl-piperazineethane-sulfonic acid buffer) is an organic buffer and very stable. In the physiological area HEPES has an excellent buffer capacity in a pH range of 7.2 to 7.6. HEPES is apt for all cell culture systems with a high proliferation and cell density. On HEPES buffered cell culture media, the incubation in a CO2 incubator is not necessary.

          Specifications:

          Sterility: Pass
          pH: 7.35-7.55
          Osmolality: 1.65-2.00 Osm/kg H20
          Particles/ml (>10µM): < 25/ml
          Particles/ml (>25µM): < 3/ml
          Color: Colorless
          Appearance: Clear

          How to use HEPES buffer:

          • dissolve powder and adjust pH at 7.2 with sodium hydroxide
          • add 10-20 ml of HEPES buffer per litre of medium (for very delicate cell cultures, use only 10 ml)
          • a concentration of 20 mM HEPES requires 20 ml/l medium, for 25 mM use 25 ml/l
          • if NaHCO3 is required, only add 10%
          Catalogue NumberL1613-BC
          Brand Family Calbiochem®
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          Ship Code Ambient Temperature Only
          Storage +2°C to +25°C
          Do not freeze Ok to freeze
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