616385 α-Toxin, Staphylococcus aureus - CAS 12616-52-3 - Calbiochem

616385
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      Overview

      Replacement Information

      Key Specifications Table

      CAS #
      12616-52-3

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      616385-250UG
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          Alu drum 250 μg
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          Description
          OverviewA monomer isolated from the Wood 46 strain of Staphylococcus aureus and purified by a modification of the methods of Harshman, et al., and Hohman. Stimulates prostaglandin synthesis and phosphorylation of myelin basic protein. Causes internucleosomal DNA degradation in T lymphocytes by forming small transmembrane pores.
          Hemolytic activity: ≥30 units/µg.
          Catalogue Number616385
          Brand Family Calbiochem®
          References
          ReferencesJonas, D., et al. 1994. Infect. Immun. 62, 1304.
          Cescatti, L., et al. 1991. J. Membr. Biol. 119, 53.
          Harshman. S., et al. 1988. Methods Enzymol. 165, 3.
          Hohman, R.J. 1988. Proc. Natl. Acad. Sci. USA 85, 1624.
          Product Information
          CAS number12616-52-3
          Unit of DefinitionOne unit is defined as the dilution of α-toxin that mediates 50% lysis of a 5% suspension of rabbit red cells.
          ATP CompetitiveN
          DeclarationNot available for sale outside of the United States.
          FormSolid
          FormulationLyophilized from 10 mM sodium phosphate buffer, pH 7.2.
          ReversibleN
          Applications
          Biological Information
          Primary TargetStimulates prostaglandin synthesis and phosphorylation of myelin basic protein.
          PuritySingle band by SDS-PAGE
          SourceA single polypeptide chain protein isolated from the Wood 46 strain of Staphylococcus aureus and purified by a modification of the methods of Harshman, S., et al. (1988) and Hohman R.J. (1988).
          Specific Activity≥30 units/µg protein
          Physicochemical Information
          Cell permeableN
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSWI0195720
          Safety Information
          R PhraseR: 26/27/28

          Very toxic by inhalation, in contact with skin and if swallowed.
          S PhraseS: 36/37/39-45

          Wear suitable protective clothing, gloves and eye/face protection.
          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Highly Toxic
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for one week at 4°C.
          End use certificateY
          Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          α-Toxin, Staphylococcus aureus - CAS 12616-52-3 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          α-Toxin, Staphylococcus aureus - CAS 12616-52-3 - Calbiochem Certificates of Analysis

          TitleLot Number
          616385

          References

          Reference overview
          Jonas, D., et al. 1994. Infect. Immun. 62, 1304.
          Cescatti, L., et al. 1991. J. Membr. Biol. 119, 53.
          Harshman. S., et al. 1988. Methods Enzymol. 165, 3.
          Hohman, R.J. 1988. Proc. Natl. Acad. Sci. USA 85, 1624.

          Technical Info

          Title
          EMD Millipore Regulated Product Information
          End Use Certificate - CWC - Regulated Products
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-October-2007 RFH
          Descriptionα-Toxin causes internucleosomal DNA degradation in T lymphocytes by forming small transmembrane pores. Many cell types are sensitive to cytolysis by α-toxin. Under certain conditions, nucleated cells can be treated with α-toxin without causing lysis. Under controlled conditions, α-toxin monomers bind to the cell membrane and form a hexamer, which functions as a hydrophilic pore with a 1-3 nm diameter. The cell becomes permeable to small molecules, while large molecules, such as enzymes or carbohydrates are excluded. The molecular weight cut-off of material passing through the pore is approximately 2-4 kDa. The pore is somewhat ion selective. Other agents commonly used for permeabilizing cells form large lesions in the membrane, making them permeable to macromolecules. α-Toxin has been utilized to introduce ions, substrates, inhibitors, nucleotides, and other pharmacological agents into cells, thereby regulating cell physiology or function. For example, the arachidonic acid cascade (and subsequent production of prostaglandin I2) is triggered by α-toxin and PMNs in endothelial cells when pores are formed that allow the passive influx of Ca2+. Stimulates prostaglandin synthesis and enhances phosphorylation of myelin basic protein.
          FormSolid
          FormulationLyophilized from 10 mM sodium phosphate buffer, pH 7.2.
          SourceA single polypeptide chain protein isolated from the Wood 46 strain of Staphylococcus aureus and purified by a modification of the methods of Harshman, S., et al. (1988) and Hohman R.J. (1988).
          CAS number12616-52-3
          RTECSWI0195720
          PuritySingle band by SDS-PAGE
          Specific activity≥30 units/µg protein
          Unit definitionOne unit is defined as the dilution of α-toxin that mediates 50% lysis of a 5% suspension of rabbit red cells.
          SolubilityReconstitute with 500 µl of sterile distilled H₂O
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for one week at 4°C.
          Toxicity Highly Toxic
          ReferencesJonas, D., et al. 1994. Infect. Immun. 62, 1304.
          Cescatti, L., et al. 1991. J. Membr. Biol. 119, 53.
          Harshman. S., et al. 1988. Methods Enzymol. 165, 3.
          Hohman, R.J. 1988. Proc. Natl. Acad. Sci. USA 85, 1624.