Protein Preparation

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Technical Resources
for Protein Preparation
Application notes, protocols, and more!

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Protein extraction and purification represent the first of many challenges in obtaining a quality lysate or purified protein sample that delivers publication-quality Western blot results. MilliporeSigma’s quality extraction kits and reagents unite superior performance with speed to reduce exposure of proteins to unfavorable conditions, leading to more stable, intact proteins for downstream analysis.

Purify Proteins

Purify your protein with PureProteome™ magnetic beads or agarose beads. PureProteome™ magnetic beads ensure fast, effective isolation of proteins without sample loss and are available in nickel, protein A, or protein G formats.

Concentrate and Desalt

Simultaneously concentrate and desalt your samples with Amicon® Ultra centrifugal filters. Their unparalleled rapid and reproducible performance minimizes protein exposure to harsh buffers. For fast and easy dialysis, use D-Tube™ Dialyzers, which provide 89% recovery and 99.9% desalting in as little as two to five hours.

Featured Extraction Kit:
ProteoExtract® Subcellular Proteome
ProteoExtract Subcellular Proteome Extraction Kit (S-PEK) is designed for fast and reproducible extraction of subcellular proteomes from adherent and suspension-grown mammalian cells.ProteoExtract Subcellular Proteome Extraction Kit (S-PEK) is designed for fast and reproducible extraction of subcellular proteomes from adherent and suspension-grown mammalian cells.

Featured Extraction Kit:
ProteoExtract® Complete Mammalian Proteome
A complete mammalian proteome extraction kit (C-PEK) designed to extract virtually all proteins from both mammalian tissues and culture cells in a simple two step process within a microcentrifuge tube to minimize protein loss.A complete mammalian proteome extraction kit (C-PEK) designed to extract virtually all proteins from both mammalian tissues and culture cells in a simple two step process within a microcentrifuge tube to minimize protein loss.