Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
If you have chosen panel analytes and then choose a premix or single plex kit, you will lose that customization.
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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You can now customize another kit, choose a premixed kit, check out or close the ordering tool.
MILLIPLEX® MAP MAPmate™ Single Plex Biomarker Assays
Designing your own multiplex assay is easy with MILLIPLEX® MAP MAPmate™ assays. Plex up to 8 individual MAPmate™ assays together using our Cell Signaling Buffer and Detection Kit (48-602MAG) or include them in existing MILLIPLEX® MAP cell signaling kits to enhance the panel or serve as controls within the guidelines provided in the protocols.
Important MAPmate™ Guidelines
Consult the protocol prior to use.
All magnetic MAPmate™ assays require the Cell Signaling Buffer & Detection Kit (48-602MAG). This kit contains all necessary reagents except the MAPmate™ assay. Both a filter and flat-bottom plate are included for convenience.
MILLIPLEX® MAP HeLa Cell Lysate:Unstimulated (Cat. # 47-205)
96-well plate and 2 sealers
96-well filter plate and 2 sealers
Each MAPmate™ includes:
Capture Antibody (bead-conjugated)
Detection Antibody (biotin-conjugated)
Positive Control (stimulated cell lysate) for phosphorylated analytes which can be used to qualify assay performance or, with serial dilutions, to develop a standard curve
3 Easy Steps to Designing a Luminex® Multiplex Signaling Assay
Step 1 Choose the analytes you want to plex together and determine their compatibility. Keep in mind that the following cannot be plexed together:
Magnetic Bead MAPmates™ and non-Magnetic Bead MAPmates™
MAPmates™ that require a different assay buffer (see buffer table subtab)
Phosphospecific and total MAPmate™ pairs (e.g. total GSK3β and GSK3β (Ser9)
Pan Tyr and phospho-specific MAPmates™ (e.g. Phsopho-EGFR (pan-Tyr) and HSP27 (Ser78)
Phospho- MAPmates™ for a single target (e.g. Akt (Ser473) and Akt (Thr308))
GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
Step 2 Order the MILLIPLEX® MAP Cell Signaling Buffer and Detection Kit (Cat. # 48-602MAG), required to run all MAPmate™ assays
Step 3 Order the MAPmate™ products you have selected.
Find and Purchase Your Favorite Analytes & Kits — Two Tools to Make Ordering Faster and Easier!
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Design & Purchase Your Own Kit
Already know your analytes of choice? Start designing and purchasing your own kits now!