The Muse® Cell Analyzer uses miniaturized fluorescence detection and microcapillary cytometry to deliver single-cell analysis, which is highly quantitative — especially when compared with traditional bulk methods like Western blot.
Sample collection, acquisition and analysis of results is achieved in less than six steps for most assays. The user interface is designed to be intuitive, so you spend less time with experimental setup and analysis for the critical cell health assays you need most.
The system delivers high-performance cell analysis using a microcapillary and miniaturized optics which occupy one-tenth the space of a typical cytometer. Laser-based fluorescence detection of each cell event can evaluate up to 3 cellular parameters. As a result, Muse® provides more quantitative results than image-based systems, which only examine up to one and two parameters, are time-consuming and provide less quantitative data.
A Basis for Sensitive Detection
Fluorescence emitted by a cellular event is detected as a voltage pulse, described in diagram to the left. The width and height are proportional to the amount of fluorescence intensity for each cell event, and this leads to highly quantitative measurements of labeled cells.
The Muse® Cell Analyzer uses patent-pending, miniaturized fluorescent detection and microcapillary technology to deliver truly accurate, precise and quantitative cell analysis compared to other methods. Laser-based fluorescence detection of each cell event can evaluate up to 3 cellular parameters – cell size (forward scatter) and 2 colors (detected in the red and/or yellow channels).
The system uses a microcapillary and miniaturized optics, which occupy one-tenth the space of a typical cytometer. This means that the instrument occupies only an 8 in x 10 in (20 cm x 25 cm) footprint. A green diode laser is used for excitation, and a uniquely designed series of retro-reflective lenses provide maximum light capture and sensitivity.
Highly Intuitive Touchscreen Interface
Muse® features a highly intuitive touchscreen interface that allows simple step-by-step operation, so easy that no flow expertise is required to run assays. The touchscreen prompts you through simple on-screen instructions and guides you though sample loading to simple setting adjustments to results—in just a few steps!
Muse® software is designed to be simple enough for any user to follow setup instructions and achieve sample acquisition, on the first use. Assay-specific software modules include sample graphs to make population gating setup foolproof. Results are displayed in both graphical and statistical formats specific to each application, making analysis unambiguous.
Muse Cell Analyzer Reported Statistics on Viable Cells.
Data output for the Muse® Count and Viability Assay. Healthy Jurkat cells were mixed with heat-killed Jurkat cells and stained with Muse® Count & Viability Reagent, and then analyzed on the Muse® Cell Analyzer. Data output include summary data (not shown) and optional dot plots (shown here). Reported statistics include viable cells/mL, % viability, and the total cells/mL. The left hand dotplot shows viability vs cell size; the right hand plot shows viability vs nucleated cells.