Molecular translocation of transcription factors from the cytoplasm to the nucleus is a pivotal event in many processes critical to cellular activation, differentiation, and host defense. Existing methods used to measure nuclear translocation have significant limitations. Amnis® imaging flow cytometry overcomes these limitations by automatic, rapid collection of images that can be objectively quantified in solution. The ability to numerically score large numbers of automatically acquired images is ideally suited to the analysis of nuclear translocation within many cell types including small, primary immune system cells.
Watch to learn how multispectral imaging in flow is being used to answer specific cell signaling research questions. Dr. Sherree Friend explains how Amnis® applications use high-throughput imaging of cell signaling events such as translocation of NFκB during inflammation. The advantages of imaging flow cytometry are compared with signaling assays that use Western blot and microscopy.