Migration & Invasion Assay Chart

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  • Cell Migration Assay Chart

    Cell Migration Assay Chart
    DescriptionPore SizePlate FormatECM CoatingDetection# TestsCat. No.
    Chemotaxis Cell Migration Assays 8 µm 24-well None Colorimetric 24 ECM508
    24-well Fluorometric 24 ECM509
    96-well Fluorometric 96 ECM510
    5 µm 24-well Colorimetric 24 ECM506
    24-well Fluorometric 24 ECM507
    96-well Fluorometric 96 ECM512
    3µm 24-well Colorimetric 24 ECM504
    24-well Fluorometric 24 ECM505
    96-well Fluorometric 96 ECM515
    Haptotaxis Cell Migration Assays 8 µm 24-well Fibronectin Colorimetric 24 ECM580
    24-well Vitronectin Fluorometric 24 ECM581
    24-well Collagen I Fluorometric 24 ECM582
    5 µm 24-well Laminin vials Colorimetric 24 ECM220
    24-well Fluorometric 24 ECM221
    Millicell® µ-Migration Assay Kit NA 4 slides of 3 wells   NA 12 MMA205
  • Cell Invasion Assay Chart

    Cell Invasion Assay Chart
    DescriptionPore SizePlate FormatECM CoatingDetection# TestsCat. No.
    Cell Invasion Assays 8 µm 24-well ECMatrix™ Colorimetric 12 ECM550
    24-well Colorimetric 24 ECM554
    96-well Colorimetric 96 ECM555
    24-well Collagen I Colorimetric 24 ECM551
    24-well Fluorometric 24 ECM552
    96-well Fluorometric 96 ECM556
    Endothelial Cell Migration Assays 3 µm 24-well Fibronectin Colorimetric 24 ECM200
    24-well Fluorometric 24 ECM201
    Endothelial Cell Invasion Assays 24-well ECMatrix™ Colorimetric 24 ECM210
    24-well Fluorometric 24 ECM211
    Leukocyte Transendothelial Migration 3 µm 24-well Fibronectin Colorimetric 24 ECM557
    Fluorometric 24 ECM559
    Tumor Cell Transendothelial Migration 8 µm 24-well Colorimetric 24 ECM558
    Fluorometric 24 ECM560
    QCM™ Invadopodia Gelatin Degradation Assay (Green) NA NA FITC-Gelatin* Fluorometric 32 ECM670
    QCM™ Invadopodia Gelatin Degradation Assay (Red)     Cy3-Gelatin* Fluorometric 32 ECM671

    * FITC-Gelatin and Cy3-Gelatin are provided but not pre-coated.

What pore size should I select?
Pore size determination depends entirely on your cell type. A quickliterature search will enable you to decide the best pore size for the particular cells you are using. The following chart illustrates pore size choice for cell lines used in our laboratory and by some of our customers for these assays.

Cell NameCell TypePore Sizes Typically UsedAssays Typically Performed
MDA-MB-231 Invasive breast cancer cell line (human) 5 or 8 µm 5 or 8 µm used in chemotaxis or invasion assay.
MCF7 Non-invasive breast cancer cell line (human) 5 or 8 µm 5 or 8 µm used in chemotaxis or invasion assay.
HT1080 Invasive fibrosarcoma cell line (human) 5 or 8 µm 5 or 8 µm used in chemotaxis or invasion assay.
NIH3T3 Non-invasive fibroblast cell line (mouse) 5 or 8 µm 5 or 8 µm used in chemotaxis or invasion assay.
HUVEC (Human vein umbilical vein endothelial cells) Endothelial cells 3 or 5 or 8 µm 3 or 5 or 8 µm in chemotaxis, invasion, angiogenesis or transendothelial migration assays.
HMVEC/HMEC (Human dermal microvascular) Endothelial cells 5 or 8 µm 5 or 8 µm in chemotaxis, invasion, angiogenesis or transendothelial migration assays.
PMN Polymorphonuclear neutrophils 1 or 3 µm 1 or 3 µm in chemotaxis assays.
Primary Stromal cells 8 µm No information available.
Epithelial cells 3 or 5 µm No information available.
Human coronary artery smooth muscle cells 5 µm No information available.
Hepatic stellate cells Myofibroblast 5 µm No information available.