Antibodies & Assays for Mitochondrial Detection
Product Highlight:MitoLight® Mitochondrial Apoptosis Detection Kit
|The MitoLight Apoptosis Detection Kit utilizes a lipophilic cation, termed as MitoLight, as a mitochondrial activity marker. MitoLight is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. MitoLight partitions differently in healthy cells than in apoptotic cells. Therefore, it has been possible to use a fluorescence ratioing technique to study mitochondrial membrane potentials. In healthy cells, the dye accumulates and aggregates in the mitochondria, giving off a bright red fluorescence (λem = 585-590 nm). In apoptotic cells with altered mitochondrial membrane potential, the dye in its monomeric form stays in the cytoplasm, fluorescing green (λem = 527-530 nm), providing a ready discrimination between apoptotic and nonapoptotic cells. The fluorescence can be observed by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using FITC channel for green monomers (Ex/Em = 488/530) and PI channel for red aggregates (Ex/Em = 488/585).
Product Highlight:FlowCellect™ MitoStress Kit
|MilliporeSigma’s FlowCellect MitoStress Kit includes MitoSOX Red, a live-cell permeant fluorogenic dye which targets the mitochondria and reacts with superoxide radicals and fluoresces yellow/red, and Annexin V conjugated to CF647 which binds to phosphatidylserine (PS) on the surface of apoptotic cells. The simultaneous use of these reagents allows researchers to obtain information on oxidative stress and apoptosis in one simple assay via flow cytometry.
Product Highlight:guava® Mitochondrial Depolarization Kit
|The guava Mitochondrial Depolarization Assay for the guava easyCyte™ Plus System allows you to monitor the loss of mitochondrial membrane potential through the use of the fluorescent cationic dye, JC-1. An optional dead cell dye, 7-AAD, is also included for discerning apoptotic cells from dead cells.
In healthy, non-apoptotic cells, the JC-1 dye enters the mitochondrial matrix, accumulates as aggregates and stains the mitochondria bright red. In apoptotic cells, the mitochondrial membrane potential collapses, and the JC-1 enters the cytoplasm as a monomeric form where it fluoresces green. Results are automatically calculated and presented in a statistical format that is easy to interpret, with total cell counts and percentages for those cell populations that are either healthy, apoptotic, or dead. For use with easyCyte Mini, easyCyte Plus.
|MitoLight Mitochondrial Apoptosis Detection Kit||APT142||25 assays|
|MitoLight Mitochondrial Apoptosis Detection Kit||APT242||100 assays|
|MTT Cell Growth Assay Kit||CT01||5000 assays|
|FlowCellect MitoStress Kit||FCCH100109||100 assays|
|FlowCellect MitoLive Kit||FCCH100107||100 assays|
|FlowCellect MitoPotential Red Kit||FCCH100105||100 assays|
|FlowCellect MitoDamage Kit||FCCH100106||100 assays|
|Guava Mitochondrial Depolarization Kit||4500-0250||100 assays|
|Mitochondria/Cytosol Fractionation Kit||MIT1000||1 Kit|
|Anti-Mitochondria, surface of intact mitochondria, clone 113-1||MAB1273||100 µL|
|Anti-Cytochrome C, clone C-7||05-479||200 µL|
|Anti-Cytochrome C, clone EP1326Y, Rabbit Monoclonal||04-1043||100 µL|
|Anti-Cytochrome c Mouse mAb (7H8.2C12)||AP1029-100UG||100 µg|
|Anti-Cytochrome c Mouse mAb (6H2.B4)||AP1030-50UG||50 µg|