Caspase Antibodies and Assays
Product Highlight:CaspaTag™ Pan-Caspase In Situ Assay Kit, Fluorescein
|MilliporeSigma's In Situ Caspase Detection Kits use a novel approach to detect active caspases. The methodology is based on Fluorochrome Inhibitors of Caspases (FLICA). The inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the inhibitor binds covalently to the active caspase.
The CaspaTag Pan-Caspase In Situ Fluorescein Assay Kit uses a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of caspase (FAM-VAD-FMK), which produces a green fluorescence. When added to a population of cells, the FAM-VAD-FMK probe enters each cell and covalently binds to a reactive cysteine residue that resides on the large subunit of the active caspase heterodimer, thereby inhibiting further enzymatic activity. The bound labeled reagent is retained within the cell, while any unbound reagent will diffuse out of the cell and is washed away. The green fluorescent signal is a direct measure of the amount of active caspase present in the cell at the time the reagent was added. Cells that contain the bound labeled reagent can be analyzed by 96-well plate-based fluorometry, fluorescence microscopy, or flow cytometry.
Product Highlight:CaspScreen Caspase Detection Kit, non-adherent cells only
|The CaspSCREEN Flow Cytometric Apoptosis Detection Kit provides a simple and convenient means for detecting activation of caspases by flow cytometry in intact cells. The assay utilizes a substrate that contains two aspartate residues linked to rhodamine 110 (D2R), a reported substrate for members of capase family proteases. The rhodamine 110 conjugated substrate is non-fluorescent, however, upon cleavage of the substrate by cellular caspases or apoptosis-related proteases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm. As the D2R substrate is more cell-permeable than other fluorometric caspase substrates, apoptosis can easily be measured in intact cells by flow cytometry.
|CaspSCREEN, non-adherent cells only||APT105||25 assays|
|CaspaTag Pan-Caspase In Situ Assay Kit, Fluorescein||APT400||100 assays|
|CaspaTag Caspase 3,7 In Situ Assay Kit, Fluorescein||APT403||100 assays|
|CaspaTag Caspase 8 In Situ Assay Kit, Fluorescein||APT408||100 assays|
|CaspaTag Caspase 9 In Situ Assay Kit, Fluorescein||APT409||100 assays|
|CaspaTag Pan-Caspase In Situ Assay Kit, Fluorescein||APT420||25 assays|
|CaspaTag Caspase 3,7 In Situ Assay Kit, Fluorescein||APT423||25 assays|
|CaspaTag Caspase 8 In Situ Assay Kit 25, Fluorescein||APT428||25 assays|
|CaspaTag Caspase 9 In Situ Assay Kit, Fluorescein||APT429||25 assays|