MAB4401 | Anti-Oct-4 Antibody, clone 10H11.2

A previous lot of this antibody was used in IC. H9 cells in culture on a mouse embronic fibroblast feeder layer were labeled by a standard indirect IF protocol. Oct-4 labelling in green, DAPI in blue, & SSEA-3 (MAB4303 in red).
A previous lot of this antibody was used in IC. H9 cells in culture on a mouse embronic fibroblast
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      Tableau de caractéristiques principal

      Espèces réactives Principales applications Hôte Format Type d'anticorps
      HumainELISA, WB, Cytométrie en flux, ImmunocytochimieSourisPurifié(e) Anticorps monoclonal
      Référence MAB4401
      Famille de marques Chemicon®
      Synonymes octamer-binding transcription factor-3 POU-type homeodomain-containing DNA-binding protein POU domain, class 5, transcription factor 1 POU domain class 5, transcription factor 1 POU class 5 homeobox 1 Octamer-binding transcription factor 3
      Nom de marque
      • Chemicon
      Description Anti-Oct-4 Antibody, clone 10H11.2
      Aperçu Purified monoclonal antibody for the detection of Human Oct-4.
      Informations générales Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
      Références bibliographiques
      Informations produit
      Format Purifié(e)
      Spécificité Reacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells).
      • Human embryonic stem cell lysate.
      Présentation Purified Ascites presented as a liquid in PBS with 0.1% sodium azide.
      Application Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2 validated for use in WB, FC, IC, ELISA.
      Principales applications
      • ELISA
      • Western Blotting
      • Cytométrie en flux
      • Immunocytochimie
      Notes opératoires Flow Cytometry: A previous lot of this antibody was used in FC.

      ELISA: A previous lot of this antibody was used in ELISA.

      Immunocytochemistry: A previous lot of this antibody was used in IC. H9 cells in culture on a mouse embronic fibroblast feeder layer were labeled by a standard indirect IF protocol. Oct-4 labelling in green, DAPI in blue, & SSEA-3 (MAB4303 in red).

      Optimal working dilutions must be determined by the end user.
      Informations biologiques
      Espèces d'immunogène Recombinant GST fusion protein, human Oct-4 (aa 1-134).
      Clone clone 10H11.2
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Hôte Souris
      Spécificité Reacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells).
      Isotype IgG1
      Espèces réactives Humain
      Species Reactivity Note The clone reacts strongly with specific nuclear binding to human ES cells and cross-reacts weakly with mouse ES cells.
      Type d'anticorps Anticorps monoclonal
      Code Entrez Gene
      Symbole du gène
      • Oct-4
      • POU5F1
      • Oct-3
      • MGC22487
      • Oct4
      • OTF4
      • OTF3
      • OCT3
      • OCT4
      Méthode de purification Protein A purfied
      Code UniProt
      Résumé UniProt FUNCTION: SwissProt: Q01860 # Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Prime candidate for an early developmental control gene.
      SIZE: 360 amino acids; 38571 Da
      TISSUE SPECIFICITY: Ubiquitous. Expressed at low levels.
      SIMILARITY: SwissProt: Q01860 ## Belongs to the POU transcription factor family. Class- 5 subfamily. & Contains 1 homeobox DNA-binding domain. & Contains 1 POU-specific domain.
      MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.
      Informations physico-chimiques
      Informations sur les matériaux
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Notifications sur l'utilisation du produit
      Assurance Qualité Evaluated by western blot on mouse P1 brain lysates.

      Western Blotting: 1:500 dilution of this antibody detected Oct-4 on 10 µg of mouse P1 brain lysates
      Notification sur l'utilisation
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Informations relatives au stockage et à l'expédition
      Conditions de stockage Stable for 1 year at from date of receipt.
      Informations sur l'emballage
      Quantité 100 µg
      Informations sur le transport
      Information complémentaire


      Certificats d'analyse

      TitreNuméro de lot
      Anti-Oct-4, clone 10H11.22474968
      Anti-Oct-4, clone 10H11.2 - 23922842392284
      Anti-Oct-4, clone 10H11.2 - 19912531991253
      Anti-Oct-4, clone 10H11.2 - 20663102066310
      Anti-Oct-4, clone 10H11.2 - LV1679970LV1679970
      Anti-Oct-4, clone 10H11.2 - LV1786447LV1786447
      Anti-Oct-4, clone 10H11.2 - NG1865606NG1865606
      Anti-Oct-4, clone 10H11.2 - NG1927045NG1927045
      Anti-Oct-4, clone 10H11.2 - PSO1553824PSO1553824
      Anti-Oct-4, clone 10H11.2 -25124862512486
      Anti-Oct-4, clone 10H11.2 -25806572580657

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      High-content imaging-based screening of microenvironment-induced changes to stem cells.
      Sebasti Vega,Er Liu,Parth J Patel,Anthony B Kulesa,Aaron L Carlson,Yanrui Ma,Matthew L Becker,Prabhas V Moghe
      Journal of biomolecular screening 17 2012

      Afficher le résumé
      Dynamic profiles of Oct-4, Cdx-2 and acetylated H4K5 in in-vivo-derived rabbit embryos.
      Chien-Hong Chen,Jie Xu,Wei-Fang Chang,Chia-Chia Liu,Hwa-Yun Su,Y Eugene Chen,Fuliang Du,Li-Ying Sung
      Reproductive biomedicine online 25 2012

      Afficher le résumé
      Oct-4B isoform is differentially expressed in breast cancer cells: hypermethylation of regulatory elements of Oct-4A suggests an alternative promoter and transcriptional start site for Oct-4B transcription.
      Wang, Yajuan, et al.
      Biosci. Rep., 31: 109-15 (2011) 2011

      Afficher le résumé
      Characterization of differential properties of rabbit tendon stem cells and tenocytes.
      Jianying Zhang,James H-C Wang
      BMC musculoskeletal disorders 11 2010

      Afficher le résumé Article en texte intégral
      Efficient derivation of embryonic stem cells from nuclear transfer and parthenogenetic embryos derived from cryopreserved oocytes.
      Sung LY, Chang CC, Amano T, Lin CJ, Amano M, Treaster SB, Xu J, Chang WF, Nagy ZP, Yang X, Tian XC
      Cell Reprogram 12 203-11. 2010

      Afficher le résumé
      Ago2 immunoprecipitation identifies predicted microRNAs in human embryonic stem cells and neural precursors.
      LA Goff, J Davila, MR Swerdel, JC Moore, RI Cohen, H Wu, YE Sun, RP Hart
      PloS one 4 e7192 2009

      Afficher le résumé Article en texte intégral
      The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used.
      Patricia A Zuk, Patricia A Zuk
      Journal of cellular biochemistry 106 867-77 2009

      Afficher le résumé
      Targeting cancer stem cells through L1CAM suppresses glioma growth.
      Bao, Shideng, et al.
      Cancer Res., 68: 6043-8 (2008) 2008

      Afficher le résumé
      Microporous membrane growth substrates for embryonic stem cell culture and differentiation.
      Steven D Sheridan,Sonia Gil,Matthew Wilgo,Aldo Pitt
      Methods in cell biology 86 2008

      Afficher le résumé


      Human Stem Cell Systems

      Informations techniques

      A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions
      Single transfection of a synthetic polycistronic self-replicative RNA yields high numbers of transgene-free human iPSCs

      Fiche technique

      Anti-Oct-4, clone 10H11.2 - Data Sheet
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      Efficient generation of transgene-free human and mouse iPS cells using a cell-permeant Tat-Cre protein

      Produits & Applications associés

      Alternative Packsize

       Référence Description  
      MAB4401-KC Anti-Oct-4, clone 10H11.2 (1mg) KC Prix & Disponibilité

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      Life Science Research > Antibodies and Assays > Primary Antibodies