Tableau de caractéristiques principal
|Species Reactivity||Key Applications||Host||Format||Antibody Type|
|H||ICC, WB, IHC||M||Purified||Monoclonal Antibody|
|Presentation||Purified mouse monoclonal IgG1 with 0.05% sodium azide.|
|Application||GCTM-5 Antibody, clone GCTM-5 is an antibody against GCTM-5 for use in IC, IH & WB.|
|Safety Information according to GHS|
|Storage and Shipping Information|
|Storage Conditions||Maintain refrigerated at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.|
|Material Size||100 µg|
|Titre||Numéro de lot|
|GCTM-5 Antibody, clone GCTM-5 - 2109546||2109546|
|GCTM-5 Antibody, clone GCTM-5 - 2283549||2283549|
|Monoclonal Antibody - LV1478901||LV1478901|
|Aperçu de la référence bibliographique||Nº PubMed|
|Transcriptional analysis of early lineage commitment in human embryonic stem cells. |
Laslett, Andrew L, et al.
BMC Dev. Biol., 7: 12 (2007) 2007
Afficher le résumé
BACKGROUND: The mechanisms responsible for the maintenance of pluripotency in human embryonic stem cells, and those that drive their commitment into particular differentiation lineages, are poorly understood. In fact, even our knowledge of the phenotype of hESC is limited, because the immunological and molecular criteria presently used to define this phenotype describe the properties of a heterogeneous population of cells. RESULTS: We used a novel approach combining immunological and transcriptional analysis (immunotranscriptional profiling) to compare gene expression in hESC populations at very early stages of differentiation. Immunotranscriptional profiling enabled us to identify novel markers of stem cells and their differentiated progeny, as well as novel potential regulators of hESC commitment and differentiation. The data show clearly that genes associated with the pluripotent state are downregulated in a coordinated fashion, and that they are co-expressed with lineage specific transcription factors in a continuum during the early stages of stem cell differentiation. CONCLUSION: These findings, that show that maintenance of pluripotency and lineage commitment are dynamic, interactive processes in hESC cultures, have important practical implications for propagation and directed differentiation of these cells, and for the interpretation of mechanistic studies of hESC renewal and commitment. Since embryonic stem cells at defined stages of commitment can be isolated in large numbers by immunological means, they provide a powerful model for studying molecular genetics of stem cell commitment in the embryo.
|Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. |
Adewumi O, et al.
Nat. Biotechnol., 25: 803-16 (2007) 2007
|Liver stem cells--prospects for clinical use. |
Tosh, David and Strain, Alastair
J. Hepatol., 42 Suppl: S75-84 (2005) 2005
|The hESC line Envy expresses high levels of GFP in all differentiated progeny. |
Costa, Magdaline, et al.
Nat. Methods, 2: 259-60 (2005) 2005
|A novel cell-surface marker found on human embryonic hepatoblasts and a subpopulation of hepatic biliary epithelial cells. |
Stamp, Lincon, et al.
Stem Cells, 23: 103-12 (2005) 2005
|Human Stem Cell Systems|